【摘要】 采用人工模拟低温环境的方法,以9a生早实核桃"香玲"和"辽核4号"离体新梢为试材,在低温胁迫下,测定细胞质膜透性、丙二醛含量、可溶性糖含量、脯氨酸含量及SOD活性,并进行生长恢复试验,用相关分析确定评价早实核桃抗寒性的生理生化指标,用隶属函数法对2个品种抗寒性进行综合评价。结果表明:随温度降低,2个品种相对电导率呈"S"型曲线;丙二醛含量呈升-降-升的趋势;可溶性糖含量先升高后降低趋势;"香玲"和"辽核4号"脯氨酸含量分别呈升-降-升-降和先升后降的趋势;"香玲"和"辽核4号"SOD活性分别呈先升后降和升-降-升-降的趋势。确定相对电导率、半致死温度、可溶性糖含量和脯氨酸含量作为评价核桃抗寒性的指标;2个品种抗寒性综合评价结果为:"辽核4号">"香玲"。更多还原

【Abstract】 The membrane permeability of 9 years old ’Xiangling’ and ’Liaohe No.4’ shoots,malondialdehyde content,the soluble sugar and praline content and SOD activity were investigated with the method of artificial simulation of low-temperature.Besides,the recovering growth situations were observed.Physiological-biochemical indicators were determined by correlation analysis and the cold resistance of the two walnut cultivars was evaluated by using fuzzy mathematics method.The results showed that the relative electric conductivity of the two walnut cultivars increased strongly with following an ’S’ shape during the drops in temperature.The malondialdehyde content increased at first,then decreased and increased again.The soluble sugar showed a tendency of increasing at the initial stage of the low temperature and decreasing later.Praline content of ’Xiangling’ increased at first,then decreased and then increased and decreased again and that of ’Liaohe No.4’ showed a tendency of increasing at first and decreasing later.SOD activity of ’Xiangling’ increased at the initial stage and decreased later and that of ’Liaohe No.4’ increased at first,then decreased and then increased and decreased again.The conductivity,semilethal temperature,the soluble sugar and praline content were determined to evaluate the cold resistance of walnut cultivars.The cold-resistance of test walnut was evaluated as ’Liaohe No.4’>’Xiangling’.更多还原