【摘要】 目的观察单味中药苦参对大鼠Ⅰ型变态反应的影响。方法以卵蛋白为变应原致敏大鼠,制备抗卵蛋白的抗血清(含IgE)。采用大鼠同种被动皮肤过敏反应(PCA)实验法,将抗血清注射到正常动物皮内,建立Ⅰ型变态反应的实验动物模型。将大鼠随机分为正常对照组(NS)、模型组(NS)、苦参高剂量(2.8×103 g/L)组、苦参低剂量(1.4×103 g/L)组、脱敏组方(2.8×103 g/L)组及酮替酚(0.4g/L)组,分别灌胃给药,然后以卵蛋白及伊文思蓝染料进行抗原攻击,测量蓝斑直径,采用分光光度计比色法测量致敏部位染料的渗出量。以抗卵蛋白血清被动致敏的大鼠颅骨骨膜肥大细胞为观察对象,从形态学角度观察苦参对肥大细胞脱颗粒的影响。结果给予高、低剂量的苦参均可抑制大鼠同种PCA,明显减少大鼠颅骨骨膜肥大细胞脱颗粒。高剂量苦参对致敏局部皮肤染料渗出和颅骨骨膜肥大细胞脱颗粒的抑制作用明显强于低剂量苦参(F=370.254、56.326,q=10.737、5.569,P<0.05);高剂量苦参的抗Ⅰ型变态反应作用与酮替酚相似(q=2.820、1.313,P>0.05),但次于脱敏组方(q=3.755、2.671,P<0.05)。结论单味中药苦参具有抗Ⅰ型变态反应的作用,呈剂量依赖性。苦参的抗Ⅰ型变态反应作用次于中药复方制剂。更多还原

【Abstract】 Objective To study the effect of Radix Sophorae Flavescentis(RSF) on type Ⅰ allergy in rats.MethodsOvalbumin was used as allergen to allergized the rat for preparing anti-ovalbumin serum containing IgE.The passive cutaneous anaphylaxis(PCA) experimentation was employed to create a model of type Ⅰ allergy in rat by intradermal injection of antiserum into the experimental animals.The rats were divided in random into normal control group,model group,high-dose(2.8×103 g/L) and low-dose(1.4×103 g/L) RSF groups,antianaphylaxis group(2.8×103 g/L) and ketotifen(0.4 g/L) group.The drugs were offered to respective groups intragastrically,the antigens were attacked by ovalbumin and Evans blue,the diameter of locus caeruleus was measured.The effusion of dye at allergized site was detected by spectrophotometer.The morphologic changes of mast cells in periost of cranial bones of rat were observed,and the influence of RSF on cell degranulation of mast cells were investigated morphologically.Results Both high and low dose of RSF inhibited PCA,obviously decreased cell degranulation of mast cells in periost of cranial bones.The inhibition of dye effusion in allergized skin and cell degranulation of mast cells in periost of cranial bones of high dose of RSF was stronger than that of low dose RSF(F=370.254,56.326;q=10.737,5.569;P<0.05).The effect of anti-type Ⅰ allergy of high dose RSF was similar to that of ketotifen(q=2.820,1.313;P>0.05),but weaker than that of antianaphylaxis group(q=3.755,2.671;P<0.05).Conclusion RSF possesses an antagonistic effect on typeⅠallergy,manifesting as dose dependent.The effect is next to compound preparation of traditional Chinese medicine.更多还原