【摘要】 [目的]提取和纯化白术多糖,并探讨多糖中蛋白对小鼠脾淋巴细胞转化的影响。[方法]采用醇沉分级、脱蛋白和Sephadex G-75制备柱从中药白术中提取分离得到cPAM、zPAM和PAM 3种多糖,并对这3种多糖进行体外淋巴细胞转化试验。[结果]3种白术多糖对ConA诱导的T淋巴细胞增殖均有促进作用;浓度为5μg/ml的zPAM对小鼠脾淋巴细胞增殖有明显的促进作用;浓度为5和10μg/ml的PAM对小鼠脾淋巴细胞增殖有明显的促进作用。[结论]白术多糖的纯化程度与脾淋巴细胞增殖有一定关系;纯化程度高的多糖对小鼠脾淋巴细胞的增殖有促进作用;白术粗多糖中的蛋白对小鼠脾淋巴细胞的增殖没有影响。更多还原

【Abstract】 [Objective] To extract and purify the polysaccharides from RHIZOMA ATRACTYLODIS MACROCEPHALAE;and to discuss the effects of polysaccharides on lymphocyte transformation of murinespleen.[Method] Polysaccharides(cPAM,zPAM,PAM) were extracted and isolated from RHIZOMA ATRACTYLODIS MACROCEPHALAE by alcohol precipitation method,deproteinization,and Sephadex G-75 preparative column.Lymphocyte transformation in vitro of the three kinds of polysaccharides was researched.[Result] The three kinds of polysaccharides all had significant promotion effects on the T lymphocyte proliferation of murinespleen induced by ConA.5 μg/ml zPAM showed significant promotion effects on the proliferation of murinespleen lymphocyte;and 5 and 10 μg/ml PAM also had significant promotion effects on the proliferation of murinespleen lymphocyte.[Conclusion] The purification degree of RHIZOMA ATRACTYLODIS MACROCEPHALAE polysaccharides had certain relationship with the proliferation of murinespleen lymphocyte.Polysaccharides with high purification degree could promote the proliferation of murinespleen lymphocyte.Crude polysaccharides from RHIZOMA ATRACTYLODIS MACROCEPHALAE had no effects on the proliferation of murinespleen lymphocyte.更多还原