【摘要】 目的探讨加味七方胃痛颗粒对慢性萎缩性胃炎(CAG)模型大鼠胃黏膜组织中胃蛋白酶含量及热休克蛋白70(HSP70)表达的影响。方法采用化学致癌物N-甲基-N謘-硝基-N-亚硝基胍(MNNG)建立CAG动物模型,以胃复春为对照组,观察加味七方胃痛颗粒对大鼠CAG相关指标的作用。结果与模型组比较,加味七方胃痛颗粒高、中剂量组和胃复春组的胃黏膜萎缩程度、胃黏膜肠化生程度明显减轻(P<0.01);加味七方胃痛颗粒高、中、低剂量组和胃复春组胃蛋白酶的含量、HSP70蛋白表达显著增加(P<0.01);与胃复春组比较,加味七方胃痛颗粒高剂量组胃蛋白酶的含量、HSP70蛋白表达显著增加(P<0.05)。结论加味七方胃痛颗粒防治CAG的作用机制可能是通过升高胃蛋白酶的含量及促进HSP70的表达,从而发挥对黏膜保护作用。更多还原

【Abstract】 Objective To investigate the effects of Modified Qifan Weitong Granules(QWG)on pepsin content and heat shock protein 70(HSP70)expression in the gastric mucosa of rats with chronic atrophic gastritis(CAG). Methods CAG rat models were established by means of N-methyl-N’s-nitro-N-nitrosoguanidine(MNNG),and the effect of QWG on relative indexes was observed and compared with that of Weifuchun Granules.Results Compared with the model group,the atrophy and intestinal metaplasia of gastric mucosa were relieved in large-and medium-dosage QWG groups and in Weifuchun Granules group,and the pepsin content and HSP70 expression were increased in large-,medium-and small-dosage QWG groups and in Weifuchun Granules group,the difference being significant(P < 0.01).Compared with Weifuchun Granules group,pepsin content and HSP70 expression in large-dosage QWG group were increased with statistical significance(P < 0.05).Conclusion The mechanism of QWG in preventing and treating CAG may be related with prevention of gastric mucosa through increasing pepsin content and HSP70 expression.更多还原