【摘要】 目的:通过脱毒快繁技术解决半夏因体内病毒积累导致种性退化和品质降低的问题。方法:以半夏叶、叶柄为外植体,采用高温处理(38℃,40 d)结合茎尖培养对半夏进行脱毒技术研究。结果:高温处理40 d左右,88.9%的植株叶片不带斑点;利用1.0 mg/L 6-BA、0.1 mg/L NAA诱导茎尖成苗,成苗率可达91.4%;MS附加0.5mg/L 6-BA、0.1 mg/L NAA有利于试管苗生长,附加0.5 mg/L KT、0.5 mg/L NAA诱导形成的单株平均根数和根长指标最好,移栽成活率可达89.5%。结论:建立了可靠的半夏脱毒快繁技术体系。更多还原

【Abstract】 Objective:To solve the degradation of production and quality of Pinellia caused by the virus accumulation,rapid propagation technical of virus-free Pinellia was researched.Methods:Pinellia leaves,petioles as explants,technology of using high temperature(38 ℃,40d) and shoot tip culture producing virus-free Pinellia was explored.Results:The results showed that leaves without virus spots was about 88.9% when explants were culture for 40d at high temperature(38℃).1.0mg/L 6-BA and 0.1mg/L NAA could induce seedling from shoot tip,seedling rate is up to 91.4%;MS added 0.5 mg/L 6-BA and 0.1mg/L NAA was conducive to growth of the plantlets;added 0.5 mg/L KT and 0.5 mg/L NAA was in favor of inducing root and promoting root growth,the survival rate of the transplanting seedling could reach 89.5%.Conclusion:A reliable system of virus-free Pinellia propagation is established.更多还原