【摘要】 目的:研究3种不同溶剂的直杆桉果实提取物体外抗单纯疱疹病毒Ⅰ型(HSV-1)和乙型肝炎病毒(HBV)的活性。方法:采用MTT法检测药物毒性,CPE法与空斑减数实验检测药物抗HSV-1活性;采用ELISA法检测药物对HepG2.2.15细胞分泌的乙型肝炎表面抗原(HBsAg)和乙型肝炎E抗原(HBeAg)的抑制作用。结果:直杆桉叶果实的醋酸乙酯提取物(P18-E1)和甲醇提取物(P18-E2)无明显抗HSV-1的活性,水提物(P18-E3)能明显抑制HSV-1的致病变作用,其TC50为69.20 mg/mLI,C50为126.77μg/mL,治疗指数为545.87;3种不同溶剂的提取物对HepG2.2.15细胞分泌的HBsAg和HBeAg均无明显抑制作用。结论:直杆桉果实的水提物(P18-E3)具有显著的抗HSV-1活性,主要是对病毒有直接灭活作用,也能影响病毒对细胞的吸附。初步推测其抗HSV-1活性的机理是影响病毒的囊膜结构从而使之失活。更多还原

【Abstract】 Objective:To investigate the antiviral activities of three kinds of extracts from the fruit of Eucalyptus maidenii against herpes simplex virus type1 and Hepatitis B Virus.Methods:Cytotoxicity of extracts on Vero cell lines were estimated using MTT method and anti-HSV-1 activity was observed and determined with CPE and plaque reduction assay.The inhibitory effects of extracts on HBsAg and HBeAg secretion in HepG2.2.15 cell culture were detected using ELISA.Results:Aqueous extract(p18-E3)had conspicuous anti-HSV-1 activity,the IC50 was 126.77 μg/mL,but the EtOAc extracts(p18-E1)and MeOH extracts(p18-E2)showed little anti-HSV-1 activity.None of these extracts had significant inhibitory eflect on HBsAg and HBeAg secretion in HepG2.2.15 cell culture.Conclusion:Aqueous extract(p18-E3)from the fruit of Eucalyptus maidenii has conspicuous anti-HSV-1 activity.It could inactivate virus directly,and inhibit virus attachment,but had no influence on virus penetration.The mechanism that p18-E3 inactivates virus might involve in viral envelope alteration.更多还原