MicroRNA Expression Patterns of the Kidney in Hyperuricemia Mice Treated with Xiezhuo Chubi Decoction(泄浊除痹方)

【摘要】 <正>Objective:To investigate the effects of Xiezhuo Chubi Decoction(泄浊除痹方,XZCBD) on the microRNA expression patterns of kidney in mice with hyperuricemia.Methods:Sixty Kunming male mice were randomly divided into the high-,medium-,and low-dose XZCBD groups,benzbromarone group,model group, and control group.Except the control group,all mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model,and the corresponding drugs(37.5 g/kg,18.75 g/kg,9.375 g/kg, and 0.02 g/kg per day) were administrated on the 7th day.On the 22nd day,the blood uric acid concentration was detected,and microRNA with obvious changes in kidney was screened with qRT-PCR.Results:The uric acid in the model group was higher than that in the control group,and the levels of the uric acid were reduced after being treated with XZCBD;the differences among groups were significant(P<0.05).Compared with the control group,32 kinds of microRNA expression changes were detected on the 15th day after being treated with high-dose XZCBD by microRNA expression profile screening.Among them,miR-34a could inhibit the expression of human urate anion exthanger 1,and miR-146a might have inhibited the inflammatory reaction.Conclusion: XZCBD could significantly reduce the serum uric acid level;its effect on hyperuricemia might be through affecting microRNA expressions.更多还原

【Abstract】 Objective:To investigate the effects of Xiezhuo Chubi Decoction(泄浊除痹方,XZCBD) on the microRNA expression patterns of kidney in mice with hyperuricemia.Methods:Sixty Kunming male mice were randomly divided into the high-,medium-,and low-dose XZCBD groups,benzbromarone group,model group, and control group.Except the control group,all mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model,and the corresponding drugs(37.5 g/kg,18.75 g/kg,9.375 g/kg, and 0.02 g/kg per day) were administrated on the 7th day.On the 22nd day,the blood uric acid concentration was detected,and microRNA with obvious changes in kidney was screened with qRT-PCR.Results:The uric acid in the model group was higher than that in the control group,and the levels of the uric acid were reduced after being treated with XZCBD;the differences among groups were significant(P<0.05).Compared with the control group,32 kinds of microRNA expression changes were detected on the 15th day after being treated with high-dose XZCBD by microRNA expression profile screening.Among them,miR-34a could inhibit the expression of human urate anion exthanger 1,and miR-146a might have inhibited the inflammatory reaction.Conclusion: XZCBD could significantly reduce the serum uric acid level;its effect on hyperuricemia might be through affecting microRNA expressions.更多还原