【摘要】 从象耳豆根结线虫中首次克隆了果胶酸裂解酶基因Me-pel-1,该基因cDNA的开放阅读框(ORF)长813 bp,编码270个氨基酸,具有果胶酸裂解酶第3家族的4个保守域特征。ME-PEL-1与南方根结线虫的果胶酸裂解酶MI-PEL-1和爪哇根结线虫的果胶酸裂解酶MJ-PEL-1相似性最高,且系统进化树显示ME-PEL-1也与它们最为接近。利用RNAi技术对象耳豆根结线虫2龄幼虫的Me-pel-1进行沉默,结果发现Me-pel-1基因被干扰后,象耳豆根结线虫2龄幼虫对番茄的侵染率显著降低。该结果表明Me-pel-1基因在象耳豆根结线虫侵染寄主的过程中发挥重要作用。更多还原

【Abstract】 Cloning and characterization of a cDNA encoding pectate lyase from the root-knot nematode Meloidogyne enterolobii is described firstly.The full length cDNA(Me-pel-1) containing a 813 bp open reading frame(ORF) that could be translated into a 270 amino acids polypeptide.The deduced protein including four conserved regions possesed characteristic of the class Ⅲ pectate lyases and showed the highest identity with MI-PEL-1 from M.incognita and MJ-PEL-1 from M.javanica.The phylogenetic tree based on the class Ⅲ pectate lyases also indicated ME-PEL-1 was the most closest to the two proteins mentioned above.Infection tests of tomato with secondary juveniles(J2s) of M.enterolobii incubated in dsRNA against Me-pel-1 resulted in lower infection efficiency,indicating the importance of the gene for plant penetration.更多还原