【摘要】 目的:建立保济丸中葛根素含量的测定方法,评价几种灭菌方法的优劣。方法:以甲醇-水(30∶70)为流动相,流速1.0 mL.min-1,检测波长250 nm,柱温30℃,用RP-HPLC测定经各种灭菌方法处理的保济丸中葛根素的含量与未经灭菌处理的保济丸中葛根素的含量。结果:葛根素在4.212～84.24 mg.L-1呈良好的线性关系(r=0.999 9),平均回收率98.18%,RSD 0.44%。湿热蒸汽灭菌方法在4种灭菌方法中属最优法。结论:定量测定方法简便、快速、灵敏、结果准确、重复性好,可作为保济丸的质量控制标准。更多还原

【Abstract】 Objective:To establish content determination method of puerarin from Baoji pills,evaluated superiority and inferior of several sterilization method.Method: In this method,methanol-water(30∶70) at flow rate of 1.0 mL·min-1 as mobile phase,detection wavelength was 250 nm at 30 ℃.Determined the content of puerarin in Baoji pills by RP-HPLC,which handled by various sterilization methods or without handling by any sterilization method.Result: Puerarin showed a good linear relationship at range of 4.212-84.24 mg·L-1(r=0.999 9).Average recovery was 98.18%,and RSD 0.44%.Heat steam sterilization method was the best method in this four methods.Conclusion: It was simple,rapid,high sensitivity and accurate with good repeatability and could be used for quality control in Baoji pills.更多还原