【摘要】 目的:建立麻藤止眩散中天麻素的含量测定方法。方法:采用高效液相色谱法(HPLC)测定麻藤止眩散中天麻素的含量。采用C18反相键合硅胶柱,以乙腈-0.1%磷酸水溶液(3∶97)为流动相,检测波长220 nm,流速1.0 mL.min-1。结果:天麻素对照品进样量在0.1～0.8μg内呈线性关系(r=0.999 8),稳定性试验RSD 0.94%,精密度试验RSD 0.76%,重复性试验RSD 1.1%,平均回收率为98.1%,RSD 1.01%。结论:该法具有操作简便、灵敏度高、重现性好等特点,可作为麻藤止眩散的质量控制方法。更多还原

【Abstract】 Objective:To determine the content of gastrodin in Mateng Zhixuan San by HPLC.Method: High performance liquid chromatography(HPLC) was appatiecl to determinae gastrodin content in Mateng Zhixuan San on RP C18 bonded with silica gel column with acetonitrile-0.1% phosphoric acid water(3∶97) as mobile phase,detection wavelength 220 nm,flow rate was 1.0 mL·min-1.Result: Gastrodin was linear in the range of 0.1-0.8 μg(r=0.999 8),RSD was 0.94%,precision test RSD was 0.76% and reproducibility test RSD was 1.1%.The average recovery was 98.1% and RSD was1.01%(n=6).Conclusion:The method is simple and sensitive for the quality control of this preparation.更多还原