【摘要】 目的:考察知母配伍黄柏对知母有效成分芒果苷在大鼠体内药物动力学的影响。方法:SD大鼠随机分为4组,分别单次灌胃(ig)芒果苷单体、知母单煎液、知母-黄柏1∶3和1∶1合煎液(剂量按芒果苷计算为35 mg·kg-1),采用液相色谱-串联质谱法测定血浆中芒果苷浓度,用DAS软件根据非房室模型法计算药物动力学参数。结果:知母单煎液组芒果苷血药浓度比单体组明显升高,配伍黄柏后有所下降。大鼠单次ig芒果苷单体、知母单煎液、知母-黄柏1∶3和1∶1合煎液后的主要药动学参数:药时曲线下面积(AUC0～t)分别为0.626,122.4,94.36,53.97 mg·L-1·h-1,峰浓度(Cmax)分别为0.149,21.52,16.26,8.27 mg·L-1,达峰时间(Tmax)分别为1.0,3.0,4.0,6.0 h,半衰期t1/2分别为3.41,1.46,1.65,1.77 h。结论:知母配伍黄柏后,芒果苷在大鼠体内的吸收随黄柏配伍比例的增加而减少,吸收速度减慢,t1/2未发生明显变化。更多还原

【Abstract】 Objective:To investigate the effects of Anemarrhenae Rhizoma combined with different proportions of Phellodendri Chinensis Cortex on the pharmacokinetics of mangiferin in rats.Method: SD rats were randomized into 4 groups and administered by single intragastric administration of mangiferin compound,Anemarrhenae Rhizoma decoction,Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex 1∶1 and 1∶3 decoctions(the dose of mangiferin:35 mg·kg-1)respectively.LC-MS-MS method was used for determination of mangiferin in rat plasma.The main pharmacokinetic parameters were calculated with DAS software by non-compartmental methods.Result: After intragastric administration of 4 formulations to the rats,the plasma concentration level of mangiferin in Anemarrhenae Rhizoma decoction group was significantly higher than that in mangiferin compound group,and was decreased by combination of Phellodendri Chinensis Cortex.The main pharmacokinetic parameters of mangiferin in 4 formulations were as follows:AUC0-t were 0.626,122.4,94.36,53.97 mg·L-1·h;Cmax were 0.149,21.52,16.26,8.27 mg·L-1;Tmax were 1.0,3.0,4.0,6.0 h;t1/2 were 3.41,1.46,1.65,1.77 h.Conclusion: Following combined with Phellodendri Chinensis Cortex,the absorption of mangiferin from Anemarrhenae Rhizoma was decreased and prolonged with the proportion increasing of Phellodendri Chinensis Cortex,and while t1/2Z showed no obvious change.更多还原