【摘要】 目的:采用高效液相色谱法测定不同产地野生滇龙胆中龙胆苦苷、獐牙菜苦苷及獐牙菜苷的含量。方法:岛津Shim-pack VP-ODS柱(4.6 mm×150 mm,5μm),流动相甲醇-水(30∶70),流速1.0 mL·min-1,龙胆苦苷检测波长270 nm,獐牙菜苦苷及獐牙菜苷检测波长245 nm,柱温25℃。结果:龙胆苦苷、獐牙菜苦苷、獐牙菜苷的线性范围为0.4～2 mg·L-1;相关系数分别为0.999 7,0.999 6,0.998 7;回收率分别为100.04%(RSD 1.49%),98.48%(RSD 1.67%),98.46%(RSD 1.15%)结论:用本法测定不同产地野生滇龙胆中3种成分含量,方法可行,结果可靠,为野生滇龙胆资源的开发与利用提供资料。更多还原

【Abstract】 Objective:To develop an HPLC method for determining the content of gentiopicroside,swertiamarin and sweroside in wild Gentiana rigescens.Method: Column was Shim-pack VP-ODS(4.6 mm×150 mm,5 μm).The mobile phase consisted of CH3OH and water(30∶70).Flow rate was 1.0 mL·min-1 at 25 ℃.Detecting wavelength of gentiopicroside was at 270 nm while swertiamarin and sweroside were detected at 245 nm.Result: The linear rang of calibration curve was 0.4-2 g·L-1 with the relation coefficient of 0.999 7,0.999 6 and 0.998 7 respectively.The average recovery of three active constituents was 100.04%(RSD 1.49%),98.48%(RSD 1.67%)and 98.46%(RSD 1.15%).Conclusion: The method is accurate and can supply basis for the exploitation and utilization of Gentiana rigescens resources.更多还原