【摘要】 采自南海软珊瑚Sarcophyton tortuosum的内生真菌Aspergillus sp.在由葡萄糖10 g/L,蛋白胨5 g/L,酵母膏2 g/L,100%海水,pH为7.5的培养基(GPY)中能够产生真菌毒素青霉酸,产量为5.5 mg/L。在GPY培养基中添加200 mg/L的单萜α-蒎烯,能改变代谢产物的组成和含量,并显著促进该真菌产生青霉酸,产量可提高至29.15 mg/L。结果表明α-蒎烯在该菌的作用下主要得到系列氧化产物,并进一步发生氧化降解。单萜α-蒎烯可以作为一个有效的诱导子,引起微生物的氧化应激反应,改变膜上酶系的活性,使代谢活动得到调控,改变代谢产物组成或产量,在微生物发酵工业上有潜在应用。更多还原

【Abstract】 Fungus Aspergillus sp.,which was isolated from soft coral Sarcophyton tortuosum,could produce mycotoxin penicillic acid with the yield of 5.5 mg/L in GPY medium containing glucose 10 g/L,peptone 5 g/L,yeast extract 2 g/L,sea water 1 L,at pH 7.5.Interestingly,while the GPY medium supplied with α-pinene at a dose of 200 mg/L,the constitute and content of the metabolites of Aspergillus sp.altered obviously.Furthermore,the yield of penicillic acid in the culture broth increased dramatically from 5.5 mg/L in control group(GPY medium) to 29.15 mg/L in biotransformation group.Based on the gas chromatography-mass spectrometry analysis,the major biotransformed products of α-pinene were characterized as oxygenated monoterpenoids,which were undergone further biodegradation.This finding suggested that monoterpene substrate,α-pinene could used to be an elicitor.It can alter the oxidase activities,trigger and modulate microbial metabolic pathways,and has potential application in fermentation.更多还原