【摘要】 目的研究HBeAg阳性慢性乙型肝炎患者HBV变异特点。方法 PCR扩增并克隆HBeAg阳性慢性乙型肝炎患者血清中HBV全基因组DNA,测序并进行基因结构分析。结果获得23株HBV全基因组DNA,它们均属于C或B基因型。与中国HBV B、C基因型参照序列相比,HBeAg阳性慢性乙型肝炎患者来源的HBV在表面抗原、P蛋白、X蛋白的反式激活区及增强子II/核心启动子区发生了一些有意义的共有变异。结论 HBV变异可能与HBeAg阳性慢性乙型肝炎的发生、发展有关。更多还原

【Abstract】 To study the characteristics of full-length Hepatitis B Virus(HBV) genomes isolated from HBeAg positive chronic hepatitis B(CHB) patients.Amplified the full-length HBV genomes by PCR from the serum of HBeAg positive CHB patients,sequenced and analyzed the structure of HBV genomes.Obtained twenty-three full-length HBV DNAs from the different CHB patients.Phylogenetic analysis revealed that all HBV strains could be categorized into genotype B or C.Structural analysis showed that,as compared to standard strains of Chinese HBV B、C subtypes,HBV obtained shared meaningful consensus mutations in B/T cell epitopes of surface and P protein,transactive domain of X protein and enhancer II/core promoter regions.Genotype and gene mutation of HBV may closely correlated with the carcinogenesis of HBV-related chronic hepatitis B.更多还原