【摘要】 目的检测并分析呼吸道疾病患者的肺炎嗜衣原体(Cpn)感染率。方法收集173例呼吸道感染患者和52例健康体检者外周血标本,分离外周血单核细胞(PBMC)血清。提取纯化PBMC的DNA,以该DNA为模板,用特异性引物体外扩增Cpn MOMP基因。扩增产物经电泳及测序鉴定,确认为Cpn特异性的目的基因。用ELISA法检测待测血清中的Cpn-IgG抗体。通过PCR与ELISA的检测结果,结合临床资料进行流行病学综合分析Cpn感染率。结果以PBMC基因组DNA为模板,呼吸道感染组患者标本中Cpn MOMP阳性率为32.40%(56/173),健康对照组中阳性率为9.62%(5/52);经测序比对,所有MOMP阳性扩增产物与Cpn AR-39的MOMP基因同源性在99%以上。ELISA检测血清Cpn-IgG,感染组阳性率50.87%(88/173),对照组阳性率为30.77%(16/52),两组差异有统计学意义。比较不同年龄组ELISA及MOMP PCR两种方法检测Cpn感染指标的阳性率,发现老年人的Cpn感染率最高。结论在呼吸道感染患者不同年龄阶段均检出较高的Cpn感染指标,其中老年人群中检出率最高,说明Cpn是本地区呼吸道感染常见的病原体之一。更多还原

【Abstract】 In order to investigate the infection rate of Chlamydophila pneumoniae,blood samples were collected from 173 respiratory tract infection patients and 52 healthy volunteers.DNA was extracted from PBMC and sera were isolated from the blood samples.The MOMP gene of C.pneumoniae were detected by PCR from the DNA samples and the C.pneumoniae specific IgG were measured by ELISA.PCR products were detected by 1.5% agarose gel electrophoresis and sequenced by Sangon Company.Combined with the clinic data,the difference of C.pneumoniae infected rate in different age group were analysed.The results of PBMC extraction were as follow: there’re 173 people in the respiratory tract infection group,and the PCR(MOMP) positive rate was 32.40%(56/173);there’re 52 people in the healthy group,and the PCR(MOMP) positive rate was 9.62%(5/52).The MOMP PCR product with Cpn AR-39 of the MOMP gene was 99% consistent.According to the positive rate of each group,the C.pneumoniae infection rate in the aged group was the highest.It’s implied that Cpn is one of the most important respiratory tract pathogen and proved to be the main pathogen for C.pneumoniae.The older people are in high-risk group for C.pneumoniae infection.更多还原