【摘要】 目的应用反相高效液相色谱法(RP-HPLC)测定羊水和胎盘中的乳酸依沙吖啶。方法 采用甲醇浸泡溶解,7%三氯乙酸沉淀蛋白的方法,提取样品后进行高效液相色谱测定。色谱柱:用Acclaim C18色谱柱(250mm×4.6mm,5μm);流动相:甲醇-乙腈-水(50∶28∶22);紫外检测器:检测波长:272nm;柱温:35℃;流速:0.8mL·min-1。结果乳酸依沙吖啶在1.0～10.0μg·mL-1与峰面积呈现良好线性关系(r=0.999 9),回收率为95.5%～99.5%,日内RSD为1.2%～1.7%,日间RSD为1.7%～2.2%。结论该方法简便、快速、灵敏、准确,可作为临床检测方法。更多还原

【Abstract】 Objective To establish an HPLC method to the monitor ethacridine lactate in the placenta and amniotic fluid. Methods The sample was dissolved in the methanol and 7% trichloroacetic acid(TDA) was used to subside the protein.The column was acclaim C18(250 mm×4.6 mm,5 μm),the mobile phase was methanol-acetonitrile-water(50∶28∶22),the wavelength was 272 nm,the column temperature was 35 ℃,and the flow rate was 0.8 mL·min-1. Results At 1.0-10.0 μg·mL-1,a good linearity(r=0.999 9) was shown.The recovery was 95.5%-99.5%,and intra-day RSD was 1.2%-1.7%,and inter-day RSD was 1.7%-2.2%. Conclusion The method is simple,rapid,sensitive,and can be used in clinical tests.更多还原