【摘要】 以MS培养基为基本培养基,添加不同种类和浓度的生长调节剂,离体培养8个马铃薯普通栽培种(2n=4x=48)品种的未授粉子房,获得了‘青薯168’和‘青薯7号’的双单体小植株。花蕾4℃下预处理24~72h的愈伤组织诱导率(51.24%~57.08%)比未预处理(9.35%)的明显提高。对‘青薯168’和‘青薯7号’而言,最佳培养基分别为MS+2-4-D2.0mg/L+ZT0.1mg/L+蔗糖30g/L+琼脂7g/L和1/2MS+GA30.5mg/L+2-4-D0.5mg/L+KT2.0mg/L+BAP2.0mg/L+ZT0.2mg/L+蔗糖20g/L+琼脂7g/L。品种对愈伤组织分化形成小植株的影响比培养基的影响要大。更多还原

【Abstract】 Calluses and dihaploid plantlets were obtained from unpollinated ovaries of ‘ Qingshu168 ’ and ‘ Qingshu7 ’ by the in vitro culture. The buds were preprocessed at 4℃ for 24-72 h, and their callus induction rate was remarkably increased, comparing with that of non-pretreatment. The most suitable differentiation medium for ‘ Qingshu168 ’ was MS + 2-4-D 2.0 mg/L + ZT 0.1 mg/L + sucrose 30 g/L + agar 7 g/L, and for ‘ Qingshu7 ’ was 1/2 MS+GA 3 0.5 mg/L+2-4-D 0.5 mg/L+KT 2.0 mg/L+BAP 2.0 mg/L+ZT 0.2 mg/L+sucrose 20 g/L+agar 7 g/L. It appeared that the plantlet differentiation mainly depended on cultivars.更多还原