【摘要】 目的:分析慢性乙型肝炎(CHB)患者CD8+T细胞TCR Vβ基因亚家族克隆化特征。方法:采用逆转录-聚合酶链反应(RT-PCR)扩增8例CHB患者外周血CD8+T细胞TCR Vβ基因22个亚家族的CDR3区,基因扫描技术对TCR Vβ亚家族的克隆化进行鉴定。结果:基因扫描显示所有8例CHB患者CD8+T细胞TCR Vβ基因亚家族均出现一个或一个以上单克隆或寡克隆增生。Vβ8、Vβ11、Vβ12出现单克隆增生的频率相对较高。8例健康者TCR Vβ基因亚家族均为多克隆。结论:CHB患者外周血CD8+T细胞TCR Vβ亚家族存在克隆性增生。更多还原

【Abstract】 Objective:To analyze clonality of TCR Vβ gene subfamilies on peripheral blood CD8+ T lymphocyte in patients with chronic hepatitis B.Methods:Expression of the 22 TCR Vβ gene subfamilies was analyzed using reverse transcription-polymerase chain reaction(RT-PCR),which amplified the complementarity determining region 3(CDR3) of TCR Vβ gene on peripheral blood CD8+T lymphocytes from 8 cases with chronic hepatitis B.PCR products were further analyzed by genescan to identify TCR Vβ subfamilies clonality.Results:Some TCR Vβ gene subfamilies showed monoclonal or oligoclonal expansion in the 8 CHB patients.Vβ8,Vβ11,Vβ12 had higher frequency of monoclonal expansion.All TCR Vβ gene families of CD8+ T lymphocyte showed a Gaussian distribution in normal control cases.Conclusion:TCR Vβ subfamilies on CD8+T lymphocyte of CHB patients during acute exacerbation showed clonal expansion.更多还原