【摘要】 目的观察八肽胆囊收缩素(CCK-8)是否能够促进周围神经损伤后的修复与再生,期待为周围神经损伤药物治疗提供一个新思路。方法健康SD大鼠随机分成2组:每组16只,其中实验组为CCK-8治疗组、对照组为生理盐水治疗组,采用右侧坐骨神经离断后缝合外膜的损伤模型。CCK-8组大鼠腹腔注射CCK-8(8nmol/kg),每天1次,连续7d,对照组则用生理盐水代替CCK-8。在术后第4周、12周观察大鼠失神经改变情况及再生神经的大体形态;步态分析测定坐骨神经功能指数恢复率(SFI%);神经电生理测定运动神经传导速度恢复率(MNCV%)和小腿腓肠肌复合动作电位波幅恢复率(CMAP%);切取损伤远端神经,半薄切片行HE及固绿髓鞘染色,观察再生神经的组织学变化;再生有髓神经纤维计数恢复率测定以检测神经再生情况;腓肠肌湿重恢复率测定CCK-8防止骨骼肌萎缩的作用。结果 (1)大体形态:术后第12周,CCK-8组再生坐骨神经粗细基本正常,对照组则较细;神经吻合口与周围组织黏连明显减轻,优于生理盐水对照组。(2)术后第4周、第12周,分别测定SFI%、MNCV%、CMAP%、再生有髓纤维计数恢复率、腓肠肌湿重恢复率,CCK-8组与对照组差异均有统计学意义。(3)组织学光镜观察:术后第4周,CCK-8组较对照组再生纤维排列规则、密集;术后第12周,CCK-8组较对照组再生纤维排列规则、均匀、髓鞘厚。结论应用CCK-8能有效地促进周围神经再生,有利于再生神经纤维传导功能及肢体运动功能的恢复,且再生纤维数量及质量优于对照组,能有效防止神经损伤后骨骼肌萎缩。更多还原

【Abstract】 Objective To observe cholecystokinin-octapeptide (CCK-8) on promoting the peripheral nervous system damage repair and the regeneration,anticipated for seek the peripheral nerve treatment medicine to provide a new mentality.Methods 32 SD rats were randomly divided into two groups:test group (CCK-8-treated group)and control group(physiological saline-treated group)with 16 in each.The right sciatic nerve was transected and sutured epineurium immediately.CCK-8 groups were injected with CCK-8(8 nmol/kg) intraperitoneally one time per day,7 days total,and control groups received isotonic saline of the same volume.After operation,each group was randomly further divided into two groups which according to the observing periods for 4 and 12 weeks respectively.Denervated changes of rat and morphologic changes of regenerated nerve were observed and recovery rate of sciatic function index(SFI%)was measured to observe the function restoration of damaged hind limbs.Recovery rate of the complex muscular action potential(CMAP%)and motor nerve conduction velocities (MNCV%) were measured with nerve-electrophysiological instrument.Specimens in the distal ends of regenerated nerves were sectioned histologically and stained with hematoxylin and eosin,firmly green myelinic membrane dyeing to observe the histomophologic changes under light microscope.Then,quantitative analysis of recovery rate of myelinated fiber populations were performed.Electron microscope was used to observe the ultrastructure changes.The recovery rate of wet weight of gastrocnemius muscle measured to observe the effect of CCK-8 on atrophy of skeletal muscles.Results (1)General morphology:at 12 weeks,the sciatic nerve of CCK-8 group was thicker than that of control group.(2)At 4 weeks and 12 weeks after operation,SFI%,MNCV%,CMAP%,the recovery rate of myelinated fiber population,the recovery rate of wet weight of gastrocnemius muscle were measured.The difference between two groups were all significant statistically.(3)The observation of light microscope and electronmicroscope:at 4 weeks,the regenerated fibers in CCK-8 group were denser and more regular than that in control group;at 12 weeks,the fibers in CCK-8 group were more regular and homogeneous and the myelin sheaths were thicker than that in control group.The observation was further confirmed under electron microscopy.Conclusions The administration of CCK-8 can effectively promotes the regeneration of peripheral nerves.After administrating CCK-8,the quantity and quality of regenerating nerves are superior to control groups,moreover,the restoration of conduction velocityes of regenerating nerve fibers and the limb functions have been improved too.CCK-8 can prevents atrophy of skeletal muscles effectively after nerve injury.更多还原