【摘要】 目的检测人脑多形性胶质瘤细胞系BT325对顺铂(DDP)、替尼泊甙(VM26)、尼莫斯汀(ACNU)、替莫唑胺(TMZ)及长春新碱(VCR)5种临床常用抗肿瘤药物的敏感性,筛选脑胶质瘤化疗敏感药物。方法用含0%、10%及20%胎牛血清的DMEM培养基培养BT325胶质瘤细胞,分别在24 h、48 h、72 h、96 h、120 h、144 h进行细胞计数,绘制细胞生长曲线,以确定细胞生长的最佳血清浓度;采用CCK-8法检测不同血浆峰浓度(PPC)5种药物对BT325胶质瘤细胞活性的影响,计算抗肿瘤药物的抑制率及IC50。结果细胞形态观察及细胞生长曲线显示含10%胎牛血清的DMEM培养基对BT325胶质瘤细胞生长较为适宜。细胞活性检测显示DDP在0.625×PPC(抑制率为51.8%)及以上浓度时可抑制BT325细胞生长,在1.25×PPC(抑制率为75.1%)及以上浓度时BT325细胞高度敏感,且有量效关系;VM26对BT325细胞抑制率均在79.6%以上,为高度敏感,剂量范围广且存在量效关系;VCR各浓度对BT325细胞抑制率为50%左右,但是与药物剂量无关;各浓度ACNU、TMZ对BT325细胞抑制率均在34%以下,无抑制作用。结论 BT325胶质瘤细胞对DDP、VM26敏感,对VCR、ACNU、TMZ不敏感。更多还原

【Abstract】 Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs,including cisplatin(DDP),teniposide(VM26),nimustine(ACNU),temozolomide(TMZ) and vincristine(VCR).Methods BT325 cells were incubat-ed in DMEM with 10% or 20% fetal bovine serum(FBS) or without FBS respectively.The cell numbers were counted at 24 h,48 h,72 h,96 h,120 h,and 144 h,then platting and growth curve were drafted.Cell counting kite-8 was used to detect the influence of 5 drugs with dif-ferent concentrations on human glioma cell line BT325.Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner,while VCR inhibited BT325 cells(50%) growth without dose-effect relationship.In contrast,ACNU and TMZ were not effective on the viability of BT325 cells.Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.更多还原