【摘要】 目的:研究并确定红花提取液的最佳醇沉工艺。方法:以羟基红花黄色素A(HSYA)转移率为评价指标进行单因素试验,分别考察终点乙醇质量分数、搅拌速度、药液初始质量浓度、醇沉温度和药液pH等因素的影响。选取对单因素试验结果影响较大的4个因素,采用L9(34)正交试验法,以HSYA转移率、浸出物得率和纯度为指标进行综合评分,确定红花提取液醇沉的最佳工艺条件。结果:红花提取液的最佳醇沉工艺条件为终点乙醇质量分数50%,药液初始质量浓度1.15 g/ml,搅拌速度500 r/min,药液pH5.0。结论:优选的工艺操作简便、稳定,HSYA转移率、浸出物得率和纯度较高。更多还原

【Abstract】 Objective: To investigate the optimal alcohol precipitation parameters for extract of Carthamus tinctorius.Methods: The effects of different factors on the transfer rate of hydroxy safflower yellow A(HSYA) was studied via single factor experiments,including the final alcohol concentration of the liquor,the speed of stirring,the initial density of the extract,the temperature and the pH of the liquor.Based on the results of single factor experiments,the final alcohol concentration of the liquor,the speed of stirring,the initial density of the extract and the pH of the liquor were studied by an orthogonal test and a multiple guidelines grading method,and the transfer rate of HSYA,the yield and the purity of extract in the supernatant were used as comprehensive evaluation index.Results: The optimal alcohol precipitation process of Carthamus tinctorius extract was as follows: the final alcohol concentration of the liquor 50%,the speed of stirring 500 r/min,the initial density of the extract 1.15 g/ml and the pH of the liquor 5.0.Conclusion: The proposed alcohol precipitation process is convenient and steady with high transfer rate of HSYA,high yield and purity of extract in the supernatant.更多还原