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液体培养法检测泌尿生殖道支原体属的效果评价

Liquid culture method in detection of mycoplasma from genitourinary tract:evaluation of effect

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【作者】 陈红霞陶日新杨振强颜卫荣

【Author】 CHEN Hong-xia,TAO Ri-xin,YANG Zhen-qiang,YAN Wei-rong (Wenling Second People′s Hospital,Wenling,Zhejiang 317502,China)

【机构】 温岭市第二人民医院检验科

【摘要】 目的用固体培养法来评价液体培养法检测泌尿生殖道支原体的效果,探讨液体培养法检测支原体属的优缺点,为临床诊断泌尿生殖道支原体属感染提供更准确的试验依据。方法采用液体培养法和固体培养法平行检测临床泌尿生殖道标本中的支原体属。结果 812例标本中液体培养法阳性361例,阳性率为44.46%,固体培养法阳性205例,阳性率为25.25%;解脲脲支原体的假阳性为59.69%,人支原体的假阳性为21.74%;两种方法差异有统计学意义(P<0.01)。结论泌尿生殖道支原体属的检测不能仅凭液体培养法,应结合固体培养法才能报告。

【Abstract】 OBJECTIVE To evaluate the liquid culture of mycoplasma detection results,discuss its advantages and disadvantages,to provide a more accurate experimental data for clinical diagnosis of mycoplasma infections. METHODS Liquid culture and solid culture were adopted to performed parallel detection of clinical specimens in the urogenital tract mycoplasma. RESULTS Liquid culture positive for 361 cases,205 cases positive for the solid culture in 812 cases.Ureaplasma urealyticum the false positive was 59.69%,Mycoplasma hominis 21.74% false positive. CONCLUSION Detection of mycoplasma can not only lean on the liquid culture,and it should report combin the result of solid culture.

  • 【文献出处】 中华医院感染学杂志 ,Chinese Journal of Nosocomiology , 编辑部邮箱 ,2011年23期
  • 【分类号】R446.5
  • 【被引频次】5
  • 【下载频次】83
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