【摘要】 目的探讨乙型肝炎病毒(HBV)前S1抗原(PreS1)、HBV-DNA、乙型肝炎病毒标志物(HBV-M)在临床中的应用价值及PreS1与病毒复制的关系。方法对583例乙型肝炎患者血清标本,采用酶联免疫法(ELISA)检测前S1抗原和乙型肝炎两对半(HBV-M),荧光PCR探针检测HBV-DNA。结果在190例HBsAg、HBeAg阳性的标本中,前S1抗原阳性为166例,HBV-DNA阳性为173例,阳性率分别为87.36%和91.05%,两种阳性率比较,差异无统计学意义;393例HBsAg阳性、HBeAg阴性的标本中,前S1抗原阳性为195例,HBV-DNA阳性为212例。阳性率分别为49.62%和53.94%,两种阳性率比较,差异无统计学意义。结论乙型肝炎病毒前S1抗原与HBV-DNA有较高的符合率,可作为判断乙型肝炎病毒早期感染、复制的敏感性指标;三者联合检测互相补充,更有助于临床疾病的诊治。更多还原

【Abstract】 OBJECTIVE To investigate the clinical significance of hepatitis B virus PreS1 antige(PreS1Ag),HBV-DNA and HBV-M and the relationship between PreS1 and virus replication.METHODS In the serum specimen of 583 cases of hepatitis B,ELISA method was used to to detect the PreS1Ag and HBV-M and FQ-PCR method was used to detect the HBV-DNA.RESULTS There were 166 cases of PreS1Ag positive and 173 cases of HBV-DNA positive in 190 cases with HBsAg and HBeAg positive,the positive rates of PreS1Ag and HBV-DNA were 87.36% and 91.05% respectively.There were 194 cases of PreS1Ag positive and 211 cases of HBV-DNA positive in 393 cases with HBsAg positive and HBeAg negative,the positive rates of PreS1Ag and HBV2DNA were 49.62% and 53.94% respectively.CONCLUSION PreS1Ag has highly coincidence with HBV-DNA.The diagnosis will be exactly if use the three methods in combination.更多还原