【摘要】 目的制备和考察姜黄素-聚乳酸/羟基乙酸纳米粒(Cur-PLGA NPs)对小鼠溃疡性结肠炎的影响。方法乳化-溶剂挥发法制备Cur-PLGA NPs,透射电镜观察外观形态,动态激光粒度分析仪分析微粒大小及分布,测定载药量。通过自由饮用5%硫酸葡聚糖钠(DSS)溶液7 d,诱导小鼠溃疡性结肠炎,考察Cur-PLGA NPs对模型动物疾病活动指数(DAI)的影响。结果姜黄素PLGA纳米粒的平均大小为(419±12)nm,载药量为(15.8±1.0)%。小鼠DAI(第7天)从小到大顺序依次为:正常组<纳米粒高剂量组<5-氨基水杨酸组<混悬液组<纳米粒中剂量组<纳米粒低剂量组<阴性对照组。结论姜黄素PLGA纳米粒对溃疡性结肠炎小鼠具有较好防治作用。更多还原

【Abstract】 AIM To prepare and investigate curcumin loaded PLGA nanoparticles(Cur-PLGA NPs) on the ulcerative colitis(UC) in mouse.METHODS Cur-PLGA-NPs were prepared by modified emulsion-solvent evaporation method.The shape and size of Cur-PLGA-NPs were observed by TEM and dynamic laser scattering analyzer,respectively.Loading capacity of drug in nanoparticles was determined.UC model mice were induced by oral administration of 5% sodium dextran sulfate(DSS) for 7 days,the pharmacodynamic effect of Cur-PLGA NPs was investigated in terms of disease active index(DAI).RESULTS The average particle size and drug loading of nanoparticles were(419±12) nm and(15.8±1.0)%,respectively.The order of DAI on the 7th day from low to high was as follows,normal <Cur-PLGA NPs-High dose < 5-ASA < Cur suspension < Cur-PLGA NPs-Middle dose < Cur-PLGA NPs-Low dose < control group.CONCLUSION Curcumine-PLGA nanoparticles displayed therapeutic and prevent effect on UC in mouse.更多还原