Effects of Er³⁺ on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro

【摘要】 A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test,alkaline phosphatase (ALP) activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effects of Er3+ on the proliferation,differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels. The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1×10-7 mol/L,but had no effect at other concentrations. Er3+ inhibited the differentiation of OBs at concentrations of 1×10-8,1×10-7,and 1×10-6 mol/L,but had no effect at a higher concentration of 1×10-5 mol/L. Er3+ had no effect on the transdifferentiation of OBs at tested concentrations. Er3+ inhibited the mineralization function of OBs at concentrations of 1×10-7,1×10-6,and 1×10-5 mol/L,but had no effect at a lower concentration of 1×10-8 mol/L. The expression of the mRNA for runt-related transcription factor 2 (RUNX-2) and peroxisome proliferators activated receptor γ (PPAR-γ) was down-regulated in the presence of 1×10-6 mol/L Er3+. These findings suggested that Er3+ might have negative effect on bone metabolism.更多还原

【Abstract】 A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test,alkaline phosphatase (ALP) activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effects of Er3+ on the proliferation,differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels. The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1×10–7 mol/L,but had no effect at other concentrations. Er3+ inhibited the differentiation of OBs at concentrations of 1×10–8,1×10–7,and 1×10–6 mol/L,but had no effect at a higher concentration of 1×10–5 mol/L. Er3+ had no effect on the transdifferentiation of OBs at tested concentrations. Er3+ inhibited the mineralization function of OBs at concentrations of 1×10–7,1×10–6,and 1×10–5 mol/L,but had no effect at a lower concentration of 1×10–8 mol/L. The expression of the mRNA for runt-related transcription factor 2 (RUNX-2) and peroxisome proliferators activated receptor γ (PPAR-γ) was down-regulated in the presence of 1×10–6 mol/L Er3+. These findings suggested that Er3+ might have negative effect on bone metabolism.更多还原