【摘要】 目的探讨低分子量肝素(LMWH)对博来霉素诱导的大鼠肺纤维化的干预作用及可能的机制。方法 100只SD雄性大鼠随机分为健康对照组、肺纤维化模型组、地塞米松干预组和LMWH干预组,每组25只。各组分别于第1,3,7,14,28d各处死5只,收集肺组织行免疫组化测定肺组织转化生长因子-β1(TGF-β1),收集血清和支气管肺泡灌洗液(BALF)行Elisa检测肺组织TGF-β1,收集肺组织检测羟脯氨酸含量。结果与模型组比较,2个干预组的肺组织TGF-β1的蛋白表达水平明显降低(P<0.05),血清和BALF的TGF-β1的蛋白表达水平明显降低(P<0.05),其中LMWH干预组较地塞米松干预组更为显著(P<0.05),与模型组比较,2个干预组的羟脯氨酸含量也明显降低(P<0.05),其中LMWH干预组较地塞米松干预组更为显著(P<0.05)。结论 LMWH具有与地塞米松相似的较强的抗纤维化效应,机制可能为通过下调细胞因子TGF-β1的表达来抑制肺纤维化。更多还原

【Abstract】 Objective To investigate the role of low molecular weight heparin(LMWH)in suppressing bleomycin-induced pulmonary fibrosis in rats.Methods One hundred SD rats were randomly divided into four groups(25 rats in each group),ie.a normal group,a bleomycin-induced pulmonary fibrosis model group,a dexamethasone-treated group,a LMWH-treated group.Five rats in each group were randomly killed on 1th day,3th day,7th day,14th day and 28th day,respectively,and lung tissue samples were harvested for histopathology study.Histoimmunochemical stain was used to determine the protein levels of transforming growth factor-β1(TGF-β1).Serum and bronchoalveolar lavage fluid(BALF)were harvested for determining the protein levels of TGF-β1 by means of Elisa,and lung tissue samples were harvested for determining concentration of hydroxyproline(HYP).Results Histoimmunochemical study suggested that both dexamethasone and LMWH could inhibit the expression of TGF-β1(compared with model group,P<0.05),there was the same tendenty of changes in serum and BALF as that in the tissue,and the suppression of TGF-β1 was more obvious in LMWH-treated group than those in dexamethasone-treated group(P<0.05).The treatment with either dexamethasone or LMWH remarkably reduced contents of HYP of lung tissue(compared with model group,P<0.05),and the suppression of contents of HYP was more obvious in LMWH-treated group than those in dexamethasone-treated group(P<0.05).Conclusion LMWH has anti-fibrosis potency similar to dexamethasone.Such effect may result from inhibition of cytokines TGF-β1.更多还原