【摘要】 本研究探讨成人急性白血病中DNA依赖蛋白激酶催化亚基(DNA-PKcs)的表达水平与临床特征、染色体及临床疗效的关系。采用间接免疫荧光法测定105例初治急性白血病患者化疗前及其中41例化疗2个疗程后骨髓单个核细胞中DNA-PKcs表达,分析其与临床特征及疗效的关系。采用R显带技术对其中26例初治白血病进行核型检测。结果表明,DNA-PKcs的表达与初诊外周血白细胞数增高有关(p<0.05),与年龄、性别、骨髓幼稚细胞比例、临床分型、平均血红蛋白水平、平均血小板计数水平无明显相关性(p>0.05)。DNA-PKcs在未缓解组中的中阳性及强阳性表达率明显高于缓解组(p<0.05)。在染色体异常组中DNA-PKcs阳性率明显高于染色体正常组(p<0.05)。结论:DNA-PKcs的表达与成人急性白血病初诊白细胞数增高相关。DNA-PKcs蛋白表达水平与急性白血病疗效、染色体异常也有相关性。更多还原

【Abstract】 This study was aimed to explore the expression of DNA-dependent protein kinase catalytic subunit(DNA-PKcs) in adult acute leukemia and its correlation with clinical characteristics,karyotype and prognosis.Indirect immuneofluorescent cytometry was used to detect the expression of DNA-PKcs in bone marrow mononuclear cells of 105 patients with acute leukemia before chemotherapy and 41 of them after 2 cycles of chemotherapy.Cytogenetic data were obtained from 26 of them by R band karyotypic analysis.The results showed that the expression of DNA-PKcs was correlated with higher WBC count level in peripheral blood(p<0.05),but was not obviously associated with median age,gender,percentage of bone marrow blasts,clinical classification,median hemoglobin level and median platelet count(p>0.05).The middle and strong positive expression of DNA-Pkcs in non-remission group was significantly higher than that in remission group(p<0.05).The positive rate of DNA-PKcs in abnormal chromosome group was significantly higher than that in chromosome normal group(p<0.05).It is concluded that the DNA-PKcs expression level is closely related with the increased WBC count,and the expression of DNA-PKcs is correlated also with karyotype and clinical prognosis in adult acute leukemia.更多还原