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紫外分光光度法测定刺五加总苷总黄酮软胶囊中总苷含量

Determination of Eleutherosides in Ciwujia Eleutherosides and Total Flavonoids Soft Capsule by UV

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【作者】 魏淑梅武桂萍王莉崔玉海李绍铭

【Author】 WEI Shu-mei,Wu Gui-ping,WANG Li,CUI Yu-hai and LI Shao-ming (Harbin Renhuang Pharmaceutical Co.,Ltd.;Key Laboratory of Chinese Herbal Extracts and Innovative Drugs of Heilongjiang Province;Ciwujia Research Institute of Heilongjiang Province,Harbin 150300,China)

【机构】 哈尔滨仁皇药业有限公司黑龙江省中药提取物及中药创新药物研究重点实验室黑龙江省刺五加研究所

【摘要】 建立紫外分光光度法测定刺五加总苷总黄酮软胶囊中总苷含量;方法:以总苷为对照品,在波长265nm处测定刺五加总苷总黄酮软胶囊中总苷含量。结果:用紫丁香苷做对照品,其线性范围20.56~102.8μg/ml,r=0.9995,其平均回收率为98.92%,加样回收率为1.5%(n=5)。结论:该方法灵敏度高,重复性好,简单,准确,是一种测定刺五加总苷总黄酮软胶囊中总苷含量的有效方法。

【Abstract】 Objective A method was proposed for the content determination of Eleutherosides in Ciwujia Eleutherosides and Total Flavonoids Soft Capsule by UV spectrophotometry.Methods The determination method was established with syringin as a contrast by UV spectrophotometry at 265 nm.Results A good linear relationship between concentration of syringin and its absorbance was obtained,and the linear range was between 20.56~102.8μg/ml with correlation coefficient of r=0.9995.The average recovery(n=5) and RSD were 98.92% and 1.5% respectively.Conclusion The method is simple,accurate and the results are stable with good reproducibility,so it can be used for the quality control of Eleutherosides in Ciwujia Eleutherosides and Total Flavonoids Soft Capsule.

【基金】 哈尔滨市科技创新人才研究专项资金项目
  • 【文献出处】 中国新技术新产品 ,China New Technologies and Products , 编辑部邮箱 ,2011年07期
  • 【分类号】R286.0
  • 【被引频次】2
  • 【下载频次】252
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