【摘要】 旨在研究DECR1基因在猪不同组织和品种中mRNA和蛋白水平的表达规律,探讨该基因与脂肪代谢的关系。以山西马身猪与大白猪为试验材料,提取肝脏、心脏、肾脏、脾脏、肺脏、胃、小肠、皮下脂肪和背最长肌组织的总RNA和总蛋白,应用实时荧光定量PCR技术检测DECR1基因在2个品种各组织中mRNA的相对表达量,采用Western blot技术对各组织中DECR1蛋白进行半定量分析。实时荧光定量PCR结果显示:DECR1基因在各组织中均有表达,组织之间的表达量存在极显著差异(P<0.01),DECR1基因在肝脏、皮下脂肪与心脏中为高丰度表达;在不同品种的皮下脂肪组织中,大白猪DECR1的mRNA表达极显著高于马身猪(P<0.01)。Western blot检测结果显示:DECR1在各组织中均有表达,不同组织的表达量存在极显著差异(P<0.01),在皮下脂肪、肝脏与小肠中高表达;不同品种的皮下脂肪组织中,大白猪DECR1蛋白的表达显著高于马身猪(P<0.05)。猪DECR1基因在不同组织的表达差异可能与脂肪代谢和脂肪沉积有关。更多还原

【Abstract】 The purpose of this study was to analyze DECR1 mRNA and protein expression profiles of Mashen and Large White pigs in different tissues,and evaluate the potential relationships between its relative expression and lipid metabolism.DECR1 expression profiles in nine tissues(liver,kidney,spleen,lung,small intestine,subcutaneous adipose,stomach,longissimus dorsi,heart) of Mashen and Large White pigs were examined by quantitative real-time PCR and Western blot,respectively.The results indicated that,by real-time PCR,DECR1 had the tissue-and species-specific in mRNA expression,but highly in liver,subcutaneous adipose and heart.The DECR1 mRNA expression in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig(P<0.01).The results showed that,by Western blot,the distribution of DECR1 in tissues were significantly different,but highly in subcutaneous adipose,liver and small intestine.Moreover,the expression of DECR1 in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig(P<0.05).It was inferred that DECR1 gene may be related to regulatory mechanism of lipid metabolism.更多还原