【摘要】 目的研究水体中二甲基甲酰胺(DMF)对东亚三角涡虫的毒性作用。方法急性毒性实验检测DMF对涡虫形态、死亡率的影响;抗氧化酶活力测定实验检测DMF对涡虫体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活力的影响;彗星实验检测DMF对涡虫DNA的损伤。结果随着DMF浓度的增加和暴露时间的延长,涡虫的死亡率呈现上升趋势;浓度高于0.1%的DMF可以抑制涡虫头部再生;DMF在0%～0.5%之间时,涡虫SOD酶活力呈"钟型"分布;0.2%～0.4%的DMF诱导CAT活力增加;但DMF对涡虫体内GSH-PX的作用规律不明显;1%的DMF对涡虫DNA有一定的损伤作用。结论 DMF对东亚三角涡虫具有明显的毒性作用;同时涡虫对DMF反应敏感,在检测水体DMF污染方面有一定的应用前景。更多还原

【Abstract】 Aim To monitor the toxic effect of N,N-dimethylformamide on the Dugesia Japonica(Planarian).Methods The effect of DMF on behavior,regeneration,mortality rate,activities of superoxidedismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX) and the damage of DNA of planarian were detected.Results The mortality rate of planarian increased obviously with the rising of DMF concentration and exposure time;the planarian regeneration was inhibited by the low dose of DMF;obvious change of SOD activity was caused by DMF ranged from 0 to 0.5%;CAT activity was increased under the treatment of DMF from 0.2% to 0.4%;DNA damage of the planarian was observed after 1% DMF treatment.Conclusion Obvious toxic effects on planarian after treatment by DMF were observed.As planarian is sensitive to DMF,it has the potentiality of DMF contamination detection in water.更多还原