【摘要】 合成了一个新的L-苯丙氨酸萘酚醛希夫碱(Naph-Phe)和邻菲咯啉(Phen)混配氧钒配合物[VO(Naph-Phe)(Phen)],通过元素分析和红外光谱进行了表征。X-射线单晶衍射分析表明,该晶体属于单斜晶系,P21/c空间群,晶胞参数为:a=1.02785(11)nm,b=2.186 5(3)nm,c=1.171 150(13)nm,β=94.747 0(10)°,V=2.622 9(4)nm3,Z=4,F(000)=1 164,R1=0.076 7,wR2=0.165 5,S=1.092。利用紫外吸收光谱、荧光光谱、圆二色光谱和粘度测量等研究了配合物与小牛胸腺DNA(CT-DNA)的作用,结果表明配合物以插入方式与CT-DNA作用。琼脂糖凝胶电泳法研究了配合物与pBR322 DNA的作用,表明它可切割超螺旋型DNA为缺刻型DNA。更多还原

【Abstract】 A new oxovanadium complex with mixed-ligands of a Schiff base(Naph-Phe) derived from L-phenylal-anine and 2-hydroxy-1-naphthaldehyde and 1,10-phenanthroline(Phen),[VO(Naph-Phe)(Phen)],has been synthe-sized and characterized by elementary analysis,IR and single crystal X-ray diffraction.It was shown that the complex crystallized in monoclinic crystal system,P21/c space group with the cell parameters: a=1.027 85(11) nm,b=2.186 5(3) nm,c=1.171 150(13) nm,β=94.747 0(10)°,V=2.622 9(4) nm3,Z=4,F(000)=1 164,R1=0.076 7,wR2=0.165 5,S=1.092.The DNA-binding properties have been investigated by UV absorption,fluorescence,CD spectra and viscosity measurement.The results indicate that the complex binds to CT-DNA in an intercalative mode.Meanwhile,the cleavage reaction on plasmid DNA has been monitored by submarine gel electrophoresis.The complex could cleave circular plasmid pBR322 DNA to nicked form.CCDC: 770157.更多还原