【摘要】 为探索高效、低污染的长春花中文多灵、长春质碱、长春新碱和长春碱的提取工艺,进行了超声波-甲醇法提取工艺条件的优化研究。结果表明:在单因素试验基础上,通过正交试验表明,超声波-甲醇法提取文多灵和长春质碱的最佳工艺条件为:提取溶剂:甲醇(pH4.0);料液比:1∶15;提取温度:45℃,提取时间:180 min,提取频率:59 KHz;文多灵和长春质碱平均得率为0.16%,0.08%。更多还原

【Abstract】 In order to explore the efficient and low contaminated extraction process of vindoline,catharanthine,vincristine and vinblastine from Catharanthus roseus,comprehensive optimization of condition in ultrasonic-methanol method were studied.On the basis of single factor experiments,orthogonal test was carried out to study.The optimum parameters for obtaining vindoline and catharanthine were determined as follows:methanol as solvent was used for 180 min,which pH value was 4.0.The ratio of raw material to solvent was 1∶15(w/v),the extraction temperature was 45 ℃,and extraction frequency was 59 KHz.The yield of vindoline and catharanthine in the conditions was 0.164% and 0.081%,respectively.更多还原