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虫草制品中核苷类有效成分分析方法研究
Research on Analytic Methods of Nucleotides in Cordyceps Preparation
【摘要】 采用HPLC法建立测定虫草中11种有效成分的分析方法。采用碳十八色谱柱,甲醇和磷酸盐水溶液进行梯度洗脱,11种有效成分分别为:胞嘧啶、尿嘧啶、次黄嘌呤、尿苷、肌苷、胸腺嘧啶、腺嘌呤、鸟苷、2’-脱氧胸苷、腺苷、虫草素。考察了方法的线性范围、稳定性以及仪器的精密度。试验数据显示上述指标均在可接受的范围内。
【Abstract】 A high performance liquid chromatographic(HPLC) method was developed for the determination of nucleotides.Meanwhile, a comparative analysis of nucleotides in Cordyceps preparation was conducted by the developed method.Results showed that a good separation of Cytosine,Uracil,Hypoxanthine,Uridine,Thymine,Adenine,Inosine,Guanidine,2’-thymidine,Adenosine, cordycepin were achieved.This method was stable,precise and reproducible and was successfully applied to the determination of nucleosides in cordyceps preparation.
【关键词】 反相高效液相色谱法;
冬虫夏草;
北冬虫夏草;
金水宝胶囊;
宁心宝胶囊;
核苷类物质;
【Key words】 HPLC; Cordyceps sinensis; Cordyceps militaris; Jinshuibao capsule; Ningxinbao capsule; nucleotides;
【Key words】 HPLC; Cordyceps sinensis; Cordyceps militaris; Jinshuibao capsule; Ningxinbao capsule; nucleotides;
【基金】 上海市科委国内合作项目(08395820200)
- 【文献出处】 天津农业科学 ,Tianjin Agricultural Sciences , 编辑部邮箱 ,2011年01期
- 【分类号】S567.35
- 【被引频次】8
- 【下载频次】150