【摘要】 目的建立气相色谱质谱(GC-MS)检测人体血液中乙醇代谢产物乙基葡萄糖醛酸苷(EtG)的方法。方法 1 ml乙二胺四乙酸二钠(EDTA-Na2)抗凝全血离心后取上层血浆,采用高氯酸去蛋白,并经氨基固相萃取小柱富集净化,提取物经BSTFA+TMCS(99∶1,v/v)衍生化,采用气相色谱-质谱法检测,总离子和选择离子模式(SIM)同时扫描,内标法定量分析。结果样品在0.2～4.0 mg/L范围内线性关系良好(0.998 8),检出限为0.17 mg/L,绝对回收率位于75.3%～85.6%之间,相对标准偏差(RSD)为5.6%～7.6%。结论该方法灵敏度高,特异性好,分析结果准确可靠,适用于法医学鉴定和临床检验中EtG的分析测定。更多还原

【Abstract】 Objective To establish a gas chromatography（GC） with mass spectrometric（MS） detection method for the determination of ethyl glucuronide（EtG）,a metabolite of alcohol in human blood. Methods After centrifugation of the 1 ml blood anticoagulated with EDTA-Na₂,the supernate was precipitated protein by perchloric acid,and then cleaned up by an NH₂-solid phase extraction cartridge.The extractions were treated with BSTFA +TMCS（99:1 v/v） for derivatization,and can be analyzed by gas chromatography-mass spectrometry in Scan and SIM mode simultaneously.The quantitation of EtG was done by using internal standard method. Results A good linearity was obtained within the concentration range of 0.2-4.0 mg /L,with the correlation coefficient of the standard curve was 0.998 8.The limit of detection（LOD） was 0.17 mg/L.The recoveries were between 75.3%-85.6%,and the relative standard deviation（RSD） were in 5.6%-7.6%. Conclusion This method is proved to be sensitive,specific,accurate,and can be applied to the determination of EtG for medicolegal identification and clinical laboratory.更多还原