【摘要】 通过观察黑曲霉菌丝体的生长及孢子萌发情况,考察壳聚糖(CS)及壳聚糖锌(CS-Zn)、壳聚糖镍(CS-Ni)的抗真菌性能。结果表明:壳聚糖、壳聚糖锌和壳聚糖镍对黑曲霉孢子萌发和菌丝体生长均具有明显抑制作用,且壳聚糖金属配合物的抑菌效果比壳聚糖本身更强,其中壳聚糖镍最强。壳聚糖镍对黑曲霉的抑菌活性受壳聚糖分子质量、环境pH值、质量浓度因素的影响较大。分子质量为5kD的CS制备的CS-Ni抗真菌效果最好;CS-Ni抑菌效果在pH3～7.5时随pH值降低而增强;随CS-Ni质量浓度(0～0.75mg/mL)的增加而增强。更多还原

【Abstract】 Chitosan and metal/chitosan complex were evaluated comparatively for their anti-Aspergillus niger activity based on the mycelial growth and spore germination of the fungus. The results showed that both chitosan and metal-chitosan could effectively inhibit the mycelial growth of A. niger and its spore germination,while chitosan displayed lower antifungal activity than its complexes with Zn or Ni,and chitosan/Ni complex showed the strongest antifungal activity. This biological activity of chitosan was largely influence by its molecular weight and concentration as well as environmental pH. The complex between chitosan with 5 kD molecular weight and Ni had the best inhibitory effect on A. niger. The inhibitory effect of chitosan/Ni complex revealed a change trend opposite to that of pH ranging from 3 to 7.5,but increased with increasing concentration from 0 to 0.75 mg/mL.更多还原