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低能N~+离子注入选育少根根霉脂肪酶高产菌株

Mutation breeding of the lipase producing strain Rhizopus arrhizus with low energy N+ion beam implantation

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【Author】 JI Yi-zhi1,2,LI Zheng3,TAN Tian-wei4,,DU Lian-xiang1,LIN Qiang2 (1.Tianjin Key Lab of Industrial Microbiology,School of Bioengineering,Tianjin University of Science and Technology,Tianjin 300222,China;2.Biochemical Engineering College of Beijing Union University,Beijing 100023,China;3.School of Textiles,Tianjin Polytechnic University,Tianjin 300160,China;4.Biorefinery Engineering Center of Ministry of Education,Beijing University of Chemical and Technology,Beijing 100029,China)

【机构】天津科技大学生物工程学院天津市工业微生物重点实验室；北京联合大学生物化学工程学院；天津工业大学-纺织学院；北京化工大学教育部生物炼制工程中心；

【摘要】以提高霉菌脂肪酶产量为目的,采用低能N+离子注入技术,对少根根霉BUCT-11进行了诱变选育研究。结果表明:经N+离子注入诱变,筛选到高产脂肪酶突变株N103,在摇瓶发酵条件下,测得脂肪酶水解酶活为175U/mL,为出发菌株产量的165%,且具有较好的遗传稳定性。更多还原

【Abstract】 In order to improve the lipase producing ability of Rhizopus arrhizus,the original strain BUCT-11 was irradiated by means of the low energy N+ ion.As a result,a lipase high-yield mutant with good genetic stability,numbered N103 was obtained,and the lipase yield of which in shaking flask scale was up to 175U/mL,which was 65% higher than that of the original strain.更多还原

【关键词】脂肪酶；少根根霉；离子注入；诱变选育；
【Key words】 lipase； Rhizopus arrhizus； ion implantation； mutation breeding；

【基金】国家“863”项目(2002AA514030);国家“十五”攻关项目(2001BA708B02208);国家自然科学基金项目(2107600C);中石化项目(202059)

【文献出处】食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2011年07期
【分类号】TQ925
【被引频次】8
【下载频次】118

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