【摘要】 本研究目的为了探讨环孢霉素A(CsA)对人早孕期母-胎界面主要组成细胞产生的IL-10与IFN-γ比率的调节作用,为治疗复发性自然流产等妊娠相关性疾病提供新线索。收集6~10周行人工流产术的正常妊娠妇女的绒毛和蜕膜组织;体外分离、培养获得蜕膜免疫细胞、蜕膜基质细胞与滋养细胞,建立三种细胞之间的两两共培养和三者共培养模型;ELISA法检测经环孢素A(0μmol/L、1μmol/L)处理48 h的共培养体系培养上清中IL-10与IFN-γ分泌水平。结果显示,(1)1μmol/L CsA可以明显促进两两共培养(P<0.05),尤其三种细胞共培养体系IL-10的分泌(P<0.01);相反,抑制蜕膜免疫细胞与滋养细胞或蜕膜基质细胞两两共培养(P<0.05),及三者共培养体系中IFN-γ的产生(P<0.05);(2)低剂量CsA升调两两共培养和三者共培养体系中IL-10与IFN-γ比率(P<0.01)。结果表明,CsA通过促进母-胎界面IL-10/IFN-γ的比率,从而有利于母-胎界面Th2型免疫优势的维持。更多还原

【Abstract】 To explore the regulatory effect of cyclosporine A(CsA) on the ratio of IL-10 to IFN-γ at the materno-fetal interface during human first trimester,which would provide evidence to treat pregnancy wastage and other pathological pregnancy.human placental and decidual tissues were collected in artificial abortion at 6～10 weeks of gestation.The decidual immuno-competent cells,trophoblast cells and decidual stromal cells were isolated by proteinase digestion and co-cultivation in vitro,and the supernatants were harvested respectively from the co-cultured cells after treatment with 1 μmol/L CsA or without CsA for 48 h.While the level of IL-10 and IFN-γ in the culture supernatants was measured by ELISA.It was found that CsA of 1μmol/L could significantly promote the secretion of IL-10 and inhibit secretion of IFN-γ in the co-cultivation of the functional cells at the maternal-fetal interface.The ratio of IL-10 to IFN-γ was significantly increased after treatment with CsA,particularly in the co-culture of all functional cells.These results suggest that CsA can improve the Th2 bias at the maternal-fetal interface in human first-trimester pregnancy.更多还原