【摘要】 目的探讨不同浓度17β-雌二醇(E2)对雌激素受体(ER)阴性的子宫内膜腺癌细胞系JEC体外生长的影响。方法选用人子宫内膜腺癌细胞系JEC进行体外培养,加入含不同浓度E2的培养液,采用MTT比色、流式细胞术和激光共聚焦扫描显微镜的方法,观察子宫内膜腺癌细胞系JEC细胞的增殖活性、细胞周期时相变化及Ca2+的变化。结果不同浓度的E2作用JEC细胞1、2、3 d后,其OD值和对照组比较无统计学差异,作用4、5 d后10-7、10-8和10-9mol/L组OD值和对照组比较差异有统计学意义(P<0.05),其中10-7mol/L组在第5天OD值与对照组比较有显著差异(P<0.01)。10-7mol/L E2作用JEC细胞4、5 d后,G0/G1、S+G2/M期细胞比例有所变化,但与对照组比较无统计学意义(P>0.05)。10-7mol/L的E2作用JEC 4 d后Ca2+的平均荧光强度值高于对照组(P<0.01)。结论 ER阴性的JEC细胞生长可受到雌激素的调控,一定浓度的雌激素对ER阴性JEC细胞生长的调控可能与细胞内Ca2+增加有关。更多还原

【Abstract】 Objective To study the effects of different concentration of estrogen(E2) on the growth of estrogen receptor-negative endometrial adenocarcinoma cell line JEC in vitro.Methods JEC was cultured in vitro.Experimental groups(E2 of different concentrations) and control group(without E2) were set up in the test.The proliferation of JEC was valuated by MTT.The cell cycle was analyzed by flow cytometry.The change of Ca2+ in JEC was examined by laser scanning confocal microscope.Results The optical density(OD) of JEC in 10-7,10-8 and 10-9 mol/L concentration of E2 at the 4th and 5th days was higher than those in control group(P<0.05).OD of JEC in 10-7 mol/L concentration of E2 at the 5th day was higher than that in control group(P<0.01).The cell cyclic distribution of JEC was unchanged than those in control group in the 4th and 5th days after dealing with 10-7 mol/L E2.The average fluorescence intensity of intracellular Ca2+ after dealing with 10-7 mol/L E2 was higher than that in control group(P<0.01).Conclusions Estrogen can stimulate the proliferation of estrogen receptor-negative JEC in vitro.It may be associated with the enhance of Ca2+.更多还原