【摘要】 目的获得产高黏度黄原胶基因工程菌株。方法利用PCR技术,以野油菜黄单胞菌X58基因组为模板扩增得到产胶基因gumBC,通过纯化、内切酶酶切、连接和接合,构建了含gumBC基因表达载体(pBBR-gumBC)的重组菌株X58-BC。结果经10 L发酵罐实验证实,gumBC过量表达,黄原胶黏度值(Brookfield s05,60 r/min)提高了62%,剪切性能值提高了57%。结论成功构建了产高黏度黄原胶基因工程菌株。更多还原

【Abstract】 Objective To obtain the recombinant strain producing high viscosity xanthan gum.Methods GumBC gene from Xanthomonas campestris X58 was cloned by PCR.The recombinant expression plasmid pBBR-gumBC was constructed by recombinant DNA technique and then conjugated into Xanthomonas campestris X58.Results The viscosity value(Brookfield s05 60r/min) and shear value of xanthan gum produced by X58-gumBC in 10L stirred fermentor after overexpression of gumBC were increased by 62 % and 57 % respectively.Conclusion The genetic engineering strain producing high viscosity xanthan gum was successfully constructed.更多还原