【摘要】 目的研究基质金属蛋白酶-1(matrix metalloproteinase-1,Mmp-1)和缺氧诱导因子-1α(Hypoxia induciblefactor-1 alpha,Hif-1α)蛋白在喉癌组织中的表达,探讨二者在喉癌发生发展中的作用及两者的相关性。方法应用免疫组化法(SP法)检测34例喉癌组织、癌旁正常组织、15例声带息肉组织中Mmp-1、Hif-1α的表达。结果①Mmp-1、Hif-1α蛋白在喉癌组织中的表达明显高于癌旁组织和息肉组织(P<0.01);癌旁组织和息肉组织间的表达差异无统计学意义(P>0.05)。②Mmp-1、Hif-1α的表达与肿瘤病理分级、临床分期、淋巴结转移有关(P<0.05),与患者的性别、年龄、吸烟量、肿瘤大小、临床分型无关(P>0.05)。③Mmp-1、Hif-1α在喉癌中的表达水平呈正相关。结论 Mmp-1和Hif-1α在喉癌组织中的高表达与喉癌的发生、发展有关,对判断喉癌的恶性程度、病情进展有重要价值。更多还原

【Abstract】 Objective To investigate the expression of matrix metalloproteinase-1(Mmp-1)and hypoxia inducible factor-1 alpha(Hif-1α)in laryngeal carcinoma and discuss their relevance and the roles in carcinogenesis and development of laryngeal carcinoma.Methods Immunohistochemical technique was used to detect the expression of Mmp-1 and Hif-1α protein in 34 tissues of laryngeal carcinoma and 34 para-carcinoma normal tissues,15 cases of vocal cord polyps.Results The expression of Mmp-1 and Hif-1α protein in laryngeal carcinoma tissues is obviously higher than that in para-carcinoma and in vocal cord polyps respectively(P<0.01),There was no significant difference between the expression of para-carcinoma and vocal cord polyps(P>0.05).The expression of Mmp-1 and Hif-1α protein was not related to patients’ age and sex,smoking history,tumor size,clinical classification(P>0.05),but to the pathological grade,clinical stage and lymph nodes metastasis(P<0.05).There was a positive correlation between the expression of Mmp-1 and Hif-1α in laryngeal carcinoma.Conclusion Overexpression of Mmp-1 and Hif-1α valuable for judgement of the malignant degree and progress of laryngeal carcinoma.was observed in laryngeal carcinoma.The high expression of Mmp-1 and Hif-1α might contribute to the carcinogenesis and development of laryngeal carcinoma,更多还原