【摘要】 [目的]获得中药三七、茯苓中克百威和抗蚜威的检测方法。[方法]通过液相色谱法对中药三七和茯苓中的克百威和抗蚜威的检测方法进行研究。[结果]确定了较优液相色谱检测条件为柱温30℃,流速1 mL/min,检测波长210 nm,流动相水-乙腈(体积比61.5∶38.5)。获得抗蚜威和克百威分别在1.99×10-4~9.95×10-4μg(r=0.9987)和1.97×10-4~9.85×10-4μg(r=0.9947)范围内线性良好。克百威和抗蚜威的RSD值分别为13.2%和7.1%。[结论]获得了克百威和抗蚜威的检测方法,并且2种中药样品中不含有克百威和抗蚜威。该方法出峰快,能避开受试中药中基质的干扰。更多还原

【Abstract】 [Aims] It was to develop the detection method of carbofuran and pirimicarb from Panax notoginseng(Burk.) F.H.Chen and Poria cocos(Schw.) Wolf.[Methods] The HPLC was employed to determine the carbofuran and pirimicarb from P.notoginseng(Burk.) F.H.Chen and P.cocos(Schw.) Wolf.[Results] The chromgraphy conditions was confirmed that the temperature was 30 °C,the velocity was 1 mL/min,the detection wavelength was 210 nm,and the mobile phase was water-acetonitrile(61.5∶38.5,by vol).The linear content of pirimicarb and carbofuran ranged from 1.99×10-4 to 9.95×10-4 μg(r=0.9987) and 1.97×10-4 to 9.85×10-4 μg(r=0.9947) respectively.The RSD of pirimicarb and carbofuran were 13.2 and 7.1% respectively.[Conclusions] The detection method was established and the two pesticides were undetected in the two Chinese herbs.The method had short retention time and the peak didn’t overlap with the base material.更多还原