【摘要】 目的:探讨CD4+CD25+Foxp3+Treg细胞在HBV感染所致慢加急性肝衰竭发病过程中的表达。方法:采用流式细胞仪检测36例慢加急性肝衰竭患者、38例慢性乙型肝炎患者、40例无症状乙肝病毒携带者及40例健康对照者外周血CD4+CD25+Foxp3+Treg细胞水平,并追踪慢加急性肝衰竭组患者转归,评价好转组与未恢复组CD4+CD25+Foxp3+Treg细胞百分率差异。结果:慢加急性肝衰竭组、慢性乙型肝炎组、无症状乙肝病毒携带组及健康对照组外周血CD4+CD25+Foxp3+Treg细胞分别为(5.14±1.03)%、(9.86±1.72)%、(7.93±1.67)%及(7.06±1.61)%,慢加急肝衰竭组外周血Treg细胞百分率显著低于慢性乙型肝炎组、无症状乙肝病毒携带组和健康对照组(P<0.01)。慢性乙型肝炎组外周血Treg百分率明显高于慢加急肝衰竭组及健康对照组,差异有统计学意义(P<0.05),而无症状乙肝病毒携带组与健康对照组外周血Treg百分率比较差异无统计学意义(P>0.05)。各组CD4+CD25+Foxp3+Treg细胞的百分率与患者血清HBVDNA呈正相关。慢加急性肝衰竭好转组与未恢复组CD4+CD25+Foxp3+Treg细胞水平比较,差异无统计学意义(P>0.05)。结论:慢加急性肝衰竭患者发病可能与CD4+CD25+Foxp3+Treg细胞表达过低有关,Treg细胞表达水平不能预示慢加急性肝衰竭患者预后。更多还原

【Abstract】 Objective: To explore the effect of CD4+CD25+Foxp3+ regulatory T cells on acute-on-chronic liver failure(ACLF) caused by hepatieis B infection.Methods: CD4+CD25+Foxp3+regulatory T cells level was detected in peripheral blood of 36 patients with ACLF(ACLF group),38 patients with chronic hepatitis B(CAH group),40 cases of asymptomatic carriers(ASC group)and 40 healthy people(control group) by flow cytometry method.The percentage of CD4+CD25+Foxp3+ regulatory T cells in non recovery group was compared with improved group of ACLF patients.Results: The expression levels of CD4+CD25+Foxp3+ regulatory T cells in peripheral blood of ACLF group,CAH group,ASC group and control group were(5.14±1.03) %,(9.86±1.72)%,(7.93±1.67) %,(7.06±1.61) % respectively.The percentage of regulatory T cells of ACLF group was significantly lower than that of CAH group,ASC group and control group(P<0.01).The percentage of peripheral blood regulatory T cells of CAH group was higher than that in ASC group and control group(P<0.05).The percentage of peripheral blood regulatory T cells of ASC group and the control group was no significant different(P> 0.05).The percentage of CD4+CD25+Foxp3+ regulatory T cells in study group was positively correlated with serum HBVDNA.Levels of CD4+CD25+Foxp3+ regulatory T cells showed no significant difference between non recovery group and improved group of ACLF patients(P> 0.05).Conclusion: The incidence of ACLF may be related with the low expression level of CD4+CD25+Foxp3+ regulatory T cells. The level of regulatory T cells couldn′t indicate the prognosis of ACLF patients.更多还原