【摘要】 目的:研究羟丙基-磺丁基-β-环糊精(HPn-SBEm-β-CD)对动物红细胞的溶血和形态改变及对动物神经、呼吸及心血管系统的影响。方法:采用紫外分光光度法检测11种HPn-SBEm-β-CD对红细胞的溶血作用;扫描电镜观察HP3-SBE2-β-CD红细胞形态的变化;给小鼠分别静脉注射80、160及320 mg.kg-1的HP4-SBE3-β-CD后对小鼠一般行为和自主活动次数等进行观察;将Beagle犬麻醉后静脉注射40、80及160 mg.kg-1的HP4-SBE3-β-CD,检测其对呼吸频率、幅度及心电、心率和血压的影响。结果:11种HPn-SBEm-β-CD溶血性均明显低于β-CD,多数化合物溶血性显著低于HP-β-CD,其中HP2-SBE3-β-CD、HP1-SBE4-β-CD、HP3-SBE6-β-CD等化合物的溶血性低于SBE7-β-CD。10 mmol.L-1HP3-SBE2-β-CD对红细胞形态无影响,而2.5 mmol.L-1 M-β-CD能明显改变红细胞的形态;给定剂量条件下HP4-SBE3-β-CD不改变小鼠的一般行为和自主活动次数;对麻醉犬的呼吸频率、幅度及心电、心率和血压均无明显影响(P>0.05)。结论:HPn-SBEm-β-CD溶血性小,对小鼠神经精神系统无影响,对麻醉犬的呼吸系统及心血管系统均无明显的影响。更多还原

【Abstract】 Objective: To investigate the effects of hydroxypropyl-sulfobutyl ether-β-cyclodextrins(HPn-SBEm-β-CD)on hemolysis and morphological changes of red blood cells(RBC) and on nervous system,respiratory system and cardiovascular system in animals. Methods: RBC were treated by 11 different degrees of substitution of HPn-SBEm-β-CD and hemolysis was detected by UV.Scanning electron microscopy(SEM) was used to observe the effect of HP3-SBE2-β-CD on morphological changes of RBC.Experimental mouse were given 80,160,and 320 mg·kg-1 HP4-SBE3-β-CD by intravenous.Different physical parameters such as common behavior,times of free activities were observed.Beagle dogs were given 40,80,and 160 mg·kg-1 HP4-SBE3-β-CD by intravenous after anaethesia and the respiratory frequency,and extent as well as ECG,blood pressure heart rate and were detected.Results: The hemolytic activity of HPn-SBEm-β-CDwas all less than β-CD.The hemolytic activity of most compounds were less than the HP-β-CD,some of the them such as HP2-SBE3-β-CD、HP1-SBE4-β-CD and HP3-SBE6-β-CD were also less than the SBE7-β-CD.10 mmol·L-1 HP3-SBE2-β-CD had no effect on morphological changes of RBC,but 2.5 mmol·L-1 M-β-CD had effect on it obviously.HP4-SBE3-β-CD of 80,160,and 320 mg·kg-1 had no effect on behavior and times of free activities.HP4-SBE3-β-CD had no obvious effect on the respiratory frequency and extent,ECG,heart rate and blood pressure.Conclusion: HPn-SBEm-β-CD with low hemolysis has no effect on the nervous system of mouse,and also has no effect on respiratory system and cardiovascular system of anesthetical dogs.更多还原