【摘要】 目的:研究大鼠涎腺放射损伤模型中NBS1基因的表达,初步探讨其在放射性涎腺上皮细胞损伤修复中的调控作用。方法:放射组40只大鼠以60Coγ射线分次照射,每次3GY,隔天1次,共5次,累积剂量分别为3、6、9、12、15 GY,对照组40只大鼠同期进行麻醉。照射后2～4 h,收集大鼠腮腺,颌下腺。应用苏木素-伊红染色(HE)和透射电镜观察涎腺组织的显微和超微结构变化;应用逆转录聚合酶链技术(RT-PCR)检测腮腺、颌下腺NBS1mRNA的表达情况。结果:腮腺较颌下腺组织损伤严重;放射组和正常组比较,腮腺放射剂量9 GY组起,颌下腺于12 GY组起,NBS1mRNA表达水平减少(P<0.05)。结论:大鼠涎腺放射损伤模型建立成功,NBS1可能参与涎腺放射损伤修复。更多还原

【Abstract】 Objective: To investigate the expression of NBS1 genes in the radiation-injured model of salivary gland in rats,and preliminary explore the irradiated injury repair mechanism of NBS1 in salivary gland.Method: The rats were randomly divided into two groups,the irradiated rats(n=40) were fractionated exposed to a dose of 3Gy 60Co γ-rays,given two days time,leading to an calculated dose of 3,6,9,12,15GY;The sham-irradiated controls(n=40) were anesthetized in parallel with the irradiated rats but not irradiated.After irradiation in two to four hours,the rats were sacrificed,the histomorphological changes were observed by HE staining;the ultrastructural changes were observed by transmission electron microscopy;RT-PCR were used to detect the NBS1expression.Result:The organization were damaged even more serious in parotid gland than in submandibular gland;Compared with the controls,with the groups of dose above 9Gy in parotid gland and the dose above 12GY in the submandibular gland,the expression of NBS1 mRNA were reduced(P < 0.05).Conclusions:The model of irradiated injury salivary gland was successfully constructed,NBS1 might be involved in salivary gland irradiated injury repair.更多还原