【摘要】 目的评价重组腺病毒介导的低氧诱导因子基因导入是否具有促进SD大鼠骨组织工程的血管化的作用。方法通过重组低氧诱导因子腺病毒载体转染SD大鼠的骨髓基质细胞,然后将含有目的基因种子细胞与骨组织工程的支架材料藻酸钙复合成可注射性组织工程骨植入裸鼠背部皮下,应用Masson染色和SP免疫组化法观察异位成骨能力,检测构建组织的微血管密度计数(MVD)。结果动物实验显示,实验组新骨形成和新生血管形成良好,实验组的微血管化密度显著地高于对照组。结论低氧诱导因子基因导入有利于组织工程骨的新骨形成和再血管化。更多还原

【Abstract】 Objective To evaluate the use of adenoviral vector of HIF-1ɑ gene for enhancing revascularization of tissue-engineered bone in SD rats.Methods The rat bone marrow stromal cells isolated from the rats’femur were transfected with HIF-1ɑ gene in vitro.The genetically modified cells were then seeded into resorbable scaffold matrix to form tissue-engineered bone those were observed by Masson staining and CD 34 immunohistochemistry.Results Tissue constructs were implanted subcutaneously into nude rats successfully.Four weeks after surgery,blood supply to the bioengineered tissue in HIF-1ɑ gene modified group was much higher than that in the control groups,and microvascular density(MVD)around the tissue-engineered bone in the experiment group significantly increased compared with the control groups.Conclusion HIF-1ɑ gene therapy mediated by adenovirus may be useful for promoting revascularization of tissue-engineered bone and enhancing new bone formation.更多还原