【摘要】 目的研究三邻甲苯基磷酸酯(TOCP)对人成神经瘤细胞(SH-SY5Y)的氧化损伤作用。方法常规方法培养SH-SY5Y细胞,经TOCP染毒,分别检测不同浓度(0~1.0 mmol/L)TOCP作用48 h以及相同浓度TOCP(0.5 mmol/L)作用不同时间后(0、24、48、72 h)细胞内丙二醛(MDA)、过氧化氢酶(CAT)的含量变化,MDA含量用硫代巴比妥酸法(TBA)检测,CAT含量用可见光分光光度法检测。结果 TOCP染毒SH-SY5Y细胞48 h的半数抑制浓度(IC50)为0.77 mmol/L。随着TOCP染毒浓度的增高,细胞内MDA的含量逐渐增高,CAT含量逐渐下降,这种变化趋势呈现明显的剂量—效应关系(P<0.05)。随着TOCP染毒时间的延长,细胞内MDA和CAT含量均呈明显的时间—效应关系。结论TOCP对SH-SY5Y细胞有明显的氧化损伤作用。更多还原

【Abstract】 Objective To study the oxidative damage of tri-ortho-cresyl phosphate(TOCP) to human neuroblastoma SH-SY5Y cells.Method The cultured SH-SY5Y cells were treated with TOCP at different concentrations(0 ~1.0 mmol/L) or at the same concentration of TOCP(0.5 mmol/L) for different time(24,48,72 h),and then,intracellular malondialdehyde(MDA) and catalase(CAT) content were detected by thiobarbituric acid method(TBA) and visible light spectrophotometry respectively.Results The half of inhibitory concentration(IC50) of TOCP treatment to SH-SY5Y cells for 48 h was 0.77 mmol/L.With the increased concentration of TOCP treatment,intracellular MDA content gradually increased,while CAT levels gradually decreased,which showed significant dose-response relationship(P<0.05).With the TOCP treatment time prolonged,intracellular MDA and CAT levels showed obvious time-effect relationship.Conclusion TOCP can cause oxidative damage on human neuroblastoma SH-SY5Y cells.更多还原