【摘要】 目的:检测河南汉族深静脉血栓形成(DVT)患者凝血因子Ⅶ(FⅦ)基因的多态性。方法:采用聚合酶链反应-限制性片段长度多态性方法检测103例河南汉族DVT患者和250名正常对照者FⅦ基因R353Q、5’F7和IVS7位点的多态性,并进行基因型频率、等位基因频率的比较及单倍型分析。结果:DVT组和对照组R353Q、5’F7基因型频率及等位基因频率差异无统计学意义(P均>0.05);2组IVS7基因型频率差异亦无统计学意义(χ2=0.327,P=0.569),DVT组H7等位基因频率(44.7%)低于对照组(51.8%)(χ2=4.112,P=0.043)。结论:FⅦ基因R353Q、5’F7多态性可能不是河南汉族人群DVT发病的遗传学风险因子;IVS7多态的H7等位基因可能是河南汉族人群DVT发病的遗传保护因子。更多还原

【Abstract】 Aim:To study the relationship between coagulation factor Ⅶ(FⅦ)gene polymorphisms and deep venous thrombosis(DVT)in Han population of Henan province.Methods:A totoal of 103 cases with DVT(patient group)and 250 age-and sex-matched healthy subjects(control group)were recruited.The R353Q,5’F7 and IVS7 polymorphisms of the coagulation FⅦ gene were detected by PCR-RFLP.Genotype frequency and allele frequency between patient group and control group were compared by SPSS software.Haplotype was analyzed by SHEsis software.Results:There were no significant difference in the genotype frequencies of R353Q or 5’F7 gene polymorphisms(P>0.05).But the IVS7/H7 allele frequency of patient group was significantly lower than that of control(44.7% vs 51.8%,χ2=4.112,P=0.043).Conclusion:The R353Q and 5’F7 polymorphisms are not associated with Henan Han population with deep venous thrombosis.The IVS7/H7 polymorphism of the factor Ⅶ gene may be protective genetic factor against deep venous thrombosis in Han population of Henan province.更多还原