【摘要】 目的:探讨锌对氟致雄性大鼠生精细胞Bcl-2和Bax蛋白表达的影响。方法:选用4～5周龄雄性Wistar大鼠50只,随机分为对照组、染氟组、氟加低锌组、氟加中锌组和氟加高锌组,灌胃染毒6周后处死动物,采用放射免疫法检测血清睾酮含量,免疫组织化学法检测生精细胞中Bcl-2和Bax蛋白的表达水平,氟离子选择电极法测定睾丸氟含量,并作精子质量分析。结果:氟加锌各剂量组血清睾酮和睾丸氟含量与对照组和染氟组比较,差异有统计学意义(F=129.179和453.812,P均<0.001)。氟加锌各剂量组Bcl-2和Bax蛋白表达水平与对照组和染氟组比较,差异有统计学意义(F=69.918和52.142,P均<0.001)。结论:锌可通过提高生精细胞Bcl-2蛋白表达、降低Bax蛋白的表达拮抗氟致大鼠生精细胞凋亡,中剂量锌拮抗作用较为明显,高剂量锌无拮抗作用甚至与氟产生协同作用。更多还原

【Abstract】 Aim:To study the effects of zinc on expressions of Bcl-2 and Bax protein caused by fluoride in spermatogenic cells of male rats.Methods:A total of 50 Wistar male rats were randomly divided into control group,fluorine treatment group,fluorine and low-dose zinc treatment group,and fluorine and middle-dose zinc treatment group,fluorine and high-dose zinc treatment group.The content of NaF in testis was measured by using fluorine selective electrode.Changes of testosterone and expressions of Bcl-2,Bax protein in spermatogenic cells were respectively observed using the methods of radioimmunoassay,immunohistochemical study.In addition,the quality of spermatozoa was observed.Results:The testosterone levels in serum of the three dose add zinc groups were higher than those of fluorine group and control group(F=129.179,P<0.001).Bcl-2 expression in spermatogenic cells of each zinc treatment groups was higher than that of fluorine treatment group(F=69.918,P<0.001).Bax expression in spermatogenic cells and the testis fluoride content of mid-dose zinc treatment group were lower than those of fluorine treatment group(F=52.142 and 453.812,P<0.001).Conclusion:Zinc could antagonize the reproductive toxicity of fluoride in male rats by increase Bcl-2 protein expression and decrease更多还原