【摘要】 目的:观察芒果苷对DHBV感染鸭脾细胞内cAMP、cGMP含量的影响。方法:建立DHBV感染的鸭乙肝动物模型,模型鸭随机分为芒果苷高、中、低剂量组及模型组,同时另设正常对照组,采用ELISA法检测各组DHBV感染鸭脾细胞内cAMP及cGMP的含量。结果:DHBV感染后,鸭脾细胞内cAMP含量明显降低(P<0.05),而cGMP含量无明显变化;芒果苷各剂量均可显著增加DHBV感染鸭脾细胞内cAMP的含量(P<0.05),但对cGMP含量的影响不明显;芒果苷中、低剂量明显提高DHBV感染鸭脾细胞内cAMP/cGMP比值(P<0.05)。结论:芒果苷可以调节DHBV感染鸭脾细胞cAMP的含量及cAMP/cGMP比值,这可能是芒果苷调节DHBV感染鸭机体免疫亢进、维持机体免疫稳定及降低肝细胞炎症发生的一条途径。更多还原

【Abstract】 Objective: To observe the mangiferin influence on cAMP, cGMP content levels in Spleen Cells of DHBV Infected Ducks. Methods:DHBV infection ducks of hepatitis B model were established, the model ducks were randomly divided into high, medium, low mangiferin dose group and model group while a separate control group was established. The cAMP, cGMP content in spleen cells of DHBV infection ducks in each group were detected by ELISA. Results: cAMP content in spleen cells of DHBV infected ducks decreased(P<0.05), while the cGMP levels had no significant change; different dose of mangiferin significantly increased the cAMP content in spleen cells of DHBV infected ducks(P<0.05),However, no significant effect on the cGMP content. The low and medium dose Mangiferin significantly improved the cAMP/cGMP ratio in spleen cells of DHBV infected ducks(P<0.05). Conclusion:Mangiferin can adjust cAMP content and cAMP/cGMP ratio in spleen cells of DHBV infected ducks, which may be the mechanism that mangiferin can regulate immune hyperthyroidism, maintain immune stability and reduce inflammation of liver cells in DHBV infected ducks.更多还原