【摘要】 目的探讨tau蛋白过度表达对细胞重新进入细胞周期的影响。方法采用免疫印迹和免疫荧光细胞化学的方法,分别检测稳定转染质粒pcDNA3.1-tau和空载体pcDNA3.1的HEK293细胞(HEK293/tau和HEK293/vec)中tau的表达,用细胞周期抑制剂Aphidicolin处理细胞抑制细胞周期,在Aphidicolin处理20h和撤药6h时应用流式细胞术检测细胞周期。结果 tau蛋白在HEK293/tau细胞中过度表达;Aphidicolin作用20h使62.33%的HEK293/tau和69.98%的HEK293/vec细胞停留在G0/G1期,两者之间差异没有统计学意义;撤药6h时,与HEK293/vec细胞相比,HEK293/tau细胞处于G0/G1期的比率显著减少,处于S期的比率显著增多。结论 Tau蛋白过度表达促进细胞重新进入细胞周期。更多还原

【Abstract】 Objective To investigate the effect of tau overexpression on the cell cycle re-entry.Methods Western blot and immunohistochemistry were used to determine the expression of tau in HEK293 stably transfected with pcDNA3.1-tau plasmid and HEK293 stably transfected with pcDNA3.1 vector(HEK293/tau and HEK293/vec).HEK293/tau and HEK293/vec cells were treated with Aphidicolin.The cell cycle distribution was detected by flow cytometry at 20 h after Aphidicolin treatment and at 6 h after Aphidicolin withdrawal respectively.Results The HEK293 cells stably transfected with the pcDNA3.1-tau plasmid expressed a high level of the tau protein in the cytoplasm.Treatment with Aphidicolin for 20 h caused 69.98% of HEK293 cells stably transfected with pcDNA3.1 vector(HEK293/vec) and 62.33% of HEK293 cells stably transfected with pcDNA3.1-tau plasmid(HEK293/tau) arrest at G0/G1 phase.Compared with HEK293/vec,the ratio of HEK293/tau cells decreased at G0/G1 phase and increased at S phase after Aphidicolin withdrawal for 6 h.Conclusion Tau protein overexpression promotes cell re-enter the cell cycle.更多还原